Selective Interaction of Colistin with Lipid Model Membranes

Biophysical Journal
Fernando G DupuyStephanie Tristram-Nagle

Abstract

Although colistin's clinical use is limited due to its nephrotoxicity, colistin is considered to be an antibiotic of last resort because it is used to treat patients infected with multidrug-resistant bacteria. In an effort to provide molecular details about colistin's ability to kill Gram-negative (G(-)) but not Gram-positive (G(+)) bacteria, we investigated the biophysics of the interaction between colistin and lipid mixtures mimicking the cytoplasmic membrane of G(+), G(-) bacteria as well as eukaryotic cells. Two different models of the G(-) outer membrane (OM) were assayed: lipid A with two deoxy-manno-octulosonyl sugar residues, and Escherichia coli lipopolysaccharide mixed with dilaurylphosphatidylglycerol. We used circular dichroism and x-ray diffuse scattering at low and wide angle in stacked multilayered samples, and neutron reflectivity of single, tethered bilayers mixed with colistin. We found no differences in secondary structure when colistin was bound to G(-) versus G(+) membrane mimics, ruling out a protein conformational change as the cause of this difference. However, bending modulus KC perturbation was quite irregular for the G(-) inner membrane, where colistin produced a softening of the membranes at an inter...Continue Reading

References

Oct 1, 1976·Immunochemistry·D C Morrison, D M Jacobs
Oct 2, 1979·Biochemistry·M Schindler, M J Osborn
Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S BrahmsA Brack
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D WadeR B Merrifield
Jan 1, 1969·Journal of Bacteriology·M KoikeT Matsuo
Jan 1, 1973·Methods in Enzymology·A J AdlerG D Fasman
Jan 1, 1984·Annual Review of Microbiology·R E Hancock
Jan 1, 1995·Advances in Microbial Physiology·R E HancockM Brown
Jun 28, 1996·The Journal of Biological Chemistry·T SteinH R Morris
Feb 8, 1997·Lancet·R E Hancock
May 29, 1999·Antimicrobial Agents and Chemotherapy·R E Hancock, D S Chapple
Nov 7, 2000·Biochimica Et Biophysica Acta·J F Nagle, S Tristram-Nagle
Apr 17, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Y LyatskayaJ F Nagle
Dec 24, 2002·Biophysical Journal·Stephanie Tristram-NagleJohn F Nagle
Jan 7, 2004·Chemistry and Physics of Lipids·Stephanie Tristram-Nagle, John F Nagle
Jun 1, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Yufeng Liu, John F Nagle
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George H TalbotUNKNOWN Antimicrobial Availability Task Force of the Infectious Diseases Society of America
Feb 17, 2006·Physical Chemistry Chemical Physics : PCCP·Yosef ScolnikMeir Shinitzky
Aug 26, 2006·The Lancet Infectious Diseases·Jian LiDavid L Paterson
Jan 19, 2007·The Journal of Physical Chemistry. B·Adrià ClausellYolanda Cajal
Apr 5, 2007·Nature Protocols·Norma J Greenfield
May 29, 2007·Biophysical Journal·Stephanie Tristram-Nagle, John F Nagle
Sep 7, 2007·Cell·Michael A KohanskiJames J Collins
Apr 9, 2008·Biophysical Journal·Thalia T MillsGerald W Feigenson
Jun 4, 2008·Physical Review Letters·Jianjun PanJohn F Nagle
Oct 2, 2008·Journal of the American Chemical Society·Richard M EpandRaquel F Epand
Oct 17, 2008·The Journal of Antimicrobial Chemotherapy·Anima PoudyalJian Li
Mar 3, 2009·Biochimica Et Biophysica Acta·Jianjun PanStephanie Tristram-Nagle
Mar 11, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·UNKNOWN Infectious Diseases Society of America

❮ Previous
Next ❯

Citations

Nov 16, 2019·Angewandte Chemie·Fang-Hsuean LiaoShu-Yi Lin
Feb 19, 2020·Communications Biology·Adree Khondker, Maikel C Rheinstädter
May 6, 2020·Pharmaceuticals·Natallia V Dubashynskaya, Yury A Skorik
Jan 10, 2020·The Journal of Physical Chemistry. B·Ana WestJeffery B Klauda
Aug 19, 2020·The Journal of Organic Chemistry·Deborah QuaglioBruno Botta
Jul 1, 2020·The Journal of Physical Chemistry. B·Ryan W LoneyStephanie A Tristram-Nagle
Sep 11, 2020·Journal of Colloid and Interface Science·Josefine Eilsø NielsenReidar Lund
Jul 3, 2021·Membranes·Vanesa Viviana Galassi, Natalia Wilke
Jul 24, 2021·International Journal of Biological Macromolecules·Natallia V DubashynskayaYury A Skorik
Aug 28, 2021·International Journal of Molecular Sciences·Natallia V DubashynskayaYury A Skorik

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.