PMID: 9434132Jan 20, 1998Paper

Selective interactions of mu-opioid receptors with pertussis toxin-sensitive G proteins: involvement of the third intracellular loop and the c-terminal tail in coupling

Biochimica Et Biophysica Acta
Z GeorgoussiG Milligan

Abstract

A cDNA encoding the rat mu-opioid receptor was expressed stably in a Rat-1 fibroblast cell line. Expression of this receptor was demonstrated with specific binding of the mu-opioid selective ligand [3H][D-Ala2,N-MePhe4,Gly5-ol]-enkephalin ([3H]DAMGO). In membranes of clone mu11 cells DAMGO produced a robust, concentration-dependent stimulation of basal high affinity GTPase activity. Cholera toxin-catalyzed [32P]ADP-ribosylation in membranes of this clone labelled a 40 kDa Gi family polypeptide(s) that was markedly enhanced by the addition of DAMGO. Antisera against Gi2alpha and Gi3alpha were both able to immunoprecipitate a [32P]-radiolabelled 40 kDa polypeptide(s) from DAMGO and cholera-toxin treated membranes of clone mu11, indicating that the mu-opioid receptor was able to interact effectively with both Gi2 and Gi3 in Rat-1 fibroblasts. A series of peptides derived from the delta-opioid receptor sequence were assessed for their ability to modify agonist-stimulated G protein activation and [3H] agonist binding to the receptor. In membranes from the clone mu11, specific binding of [3H]DAMGO was reduced by peptides corresponding to the NH2-terminal region of the third intracellular loop (i3.1) and the carboxyl-terminal tail (i4...Continue Reading

References

Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B L KiefferC G Hirth
Dec 28, 1992·Science·C J EvansR H Edwards
Jan 1, 1991·Life Sciences·S R Childers
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S CotecchiaR J Lefkowitz
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B KönigK P Hofmann
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H UedaH Takagi
Aug 1, 1983·Computer Programs in Biomedicine·G A McPherson
Mar 1, 1994·Trends in Neurosciences·G R UhlG Pasternak
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·J LiuJ Wess

❮ Previous
Next ❯

Citations

Nov 2, 2005·Neurotoxicity Research·K F HauserP E Knapp
Nov 22, 2011·Life Sciences·Lorella PasquinucciGiuseppe Ronsisvalle
May 4, 2010·Neuropharmacology·Eirini-Maria GeorgantaZafiroula Georgoussi
Aug 3, 2013·Journal of Neurochemistry·Eirini-Maria GeorgantaZafiroula Georgoussi
Apr 29, 2005·Journal of Neurochemistry·Georgia Mazarakou, Zafiroula Georgoussi
Feb 18, 2014·British Journal of Pharmacology·Alisa Knapman, Mark Connor
May 16, 2012·Biochemical and Biophysical Research Communications·Brian F O'DowdSusan R George
Jan 8, 2015·Frontiers in Pharmacology·Stéphane AlloucheNicolas Marie
May 14, 2008·Biochemical and Biophysical Research Communications·Yoshiaki YanoKatsumi Matsuzaki
Sep 15, 2005·The Journal of Pharmacology and Experimental Therapeutics·Evangelia Morou, Zafiroula Georgoussi
Mar 11, 2003·The Journal of Pharmacology and Experimental Therapeutics·Vipa ChaipatikulP Y Law
Jun 20, 2003·Molecular Pharmacology·Vipa ChaipatikulPing-Yee Law
Sep 21, 2000·The Journal of Biological Chemistry·S M AppleyardC Chavkin
Mar 22, 2001·Journal of Neurochemistry·H E MoonG Milligan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.