Selective isolation of allergens. I. Reaction of house dust mite extracts with tridacnin and concanavalin A and examination of the allergenicity of the isolated components

Clinical Allergy
B A Baldo, G Uhlenbruck

Abstract

Tridacnin, a lectin from the clam Tridacna maxima was found to precipitate with crude extracts from the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus. Using tridacnin in direct precipitation and affinity chromatography studies, a carbohydrate-rich preparation of high molecular weight was isolated from D. farinae extracts. The isolated preparation gave one precipitin line when used with tridacnin in gel diffusion and immunoelectrophoresis experiments but two bands were seen on polyacrylamide disc gels after electrophoresis at pH 8.9. Radioallergosorbent test (RAST) studies demonstrated that the isolated material reacted strongly with sera from human subjects allergic to house dust mites and accounted for a significant proportion of the IgE binding capacity of the crude D. farinae extracts. Only one out of fifteen mite-allergic subjects gave a positive response when prick tested with the isolated preparation at a concentration of 0.2 mg/ml. A possible explanation for the discrepancy observed between RAST and skin test results is discussed. Concanavalin A also precipitated with D. farinae extracts but the material isolated with this lectin did not react with tridacnin and reacted weakly in the RAST w...Continue Reading

References

Apr 9, 1977·Lancet·B A Baldo, G Uhlenbruck
Jan 1, 1976·Advances in Immunology·T P King
Jan 1, 1975·Advances in Experimental Medicine and Biology·B A Baldo, G Uhlenbruck
Nov 18, 1976·The New England Journal of Medicine·W J MetzgerD Levitz
Jan 1, 1976·International Archives of Allergy and Applied Immunology·S RomagnaniM Ricci
Jun 15, 1968·Lancet·K MaunsellA M Cunnington
Jun 15, 1968·Lancet·J PepysF E Hargreave
Jun 1, 1970·The Journal of Allergy·H BaerL M Lichtenstein
Sep 1, 1974·Archives of Disease in Childhood·J K Sarsfield
Jan 1, 1972·The Journal of Allergy and Clinical Immunology·M CeskaJ M Varga
Jan 1, 1973·Annual Review of Biochemistry·H Lis, N Sharon
Jan 1, 1971·International Archives of Allergy and Applied Immunology·S Elsayed, K Aas
Sep 15, 1972·Science·N Sharon, H Lis
Mar 1, 1964·Biochemistry·T P KINGJ T CONNELL
Jun 7, 2007·Neurorehabilitation and Neural Repair·Hans-Otto Karnath, Doris Brötz

Citations

Sep 1, 1979·Die Naturwissenschaften·S KrilisA Basten
Mar 1, 1982·Clinical Allergy·B A BaldoK M Taylor
Aug 31, 2019·BioMed Research International·Rafael de Oliveira ResendeErnesto Akio Taketomi

Related Concepts

Allergens
Concanavalin A
Allergic Reaction
Immunotherapy
Acarus
Radioallergosorbent Test
Hypersensitivity Skin Testing
Animal Lectins

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.