Selective isolation of mammalian genes by TAR cloning

Current Protocols in Human Genetics
N Kouprina, V Larionov

Abstract

Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShizuyaM Simon
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D T StinchcombR W Davis
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·V LarionovM A Resnick
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·V LarionovM A Resnick
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·V LarionovM A Resnick
Oct 23, 1997·Gene·N KouprinaV Larionov
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J F Theis, C S Newlon
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N KouprinaV Larionov
Aug 13, 2003·Journal of Molecular Evolution·Vladimir N NoskovVladimir Larionov
Nov 26, 2003·FEMS Microbiology Reviews·Natalay Kouprina, Vladimir Larionov
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Natalay KouprinaVladimir Larionov
Sep 24, 2004·Human Molecular Genetics·Adam PavlicekVladimir Larionov

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Citations

Jan 21, 2011·BMC Genomics·Mariana C SimõesGuilherme Oliveira

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