Selective killing of cancer cells by leaf extract of Ashwagandha: components, activity and pathway analyses

Cancer Letters
Nashi WidodoRenu Wadhwa

Abstract

Ashwagandha, also called as "Queen of Ayurveda" and "Indian ginseng", is a commonly used plant in Indian traditional medicine, Ayurveda. Its roots have been used as herb remedy to treat a variety of ailments and to promote general wellness. However, scientific evidence to its effects is limited to only a small number of studies. We had previously identified anti-cancer activity in the leaf extract (i-Extract) of Ashwagandha and demonstrated withanone as a cancer inhibitory factor (i-Factor). In the present study, we fractionated the i-Extract to its components by silica gel column chromatography and subjected them to cell based activity analyses. We found that the cancer inhibitory leaf extract (i-Extract) has, at least, seven components that could cause cancer cell killing; i-Factor showed the highest selectivity for cancer cells and i-Factor rich Ashwagandha leaf powder was non-toxic and anti-tumorigenic in mice assays. We undertook a gene silencing and pathway analysis approach and found that i-Extract and its components kill cancer cells by at least five different pathways, viz. p53 signaling, GM-CFS signaling, death receptor signaling, apoptosis signaling and G2-M DNA damage regulation pathway. p53 signaling was most commo...Continue Reading

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Citations

Sep 3, 2013·Journal of Biotechnology·Varsha P Wadegaonkar, Prasad A Wadegaonkar
Dec 17, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Hardeep KatariaGurcharan Kaur
Jul 10, 2009·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Nashi WidodoRenu Wadhwa
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Oct 24, 2009·Chinese Medicine·Hongwei WangJingtian Xie
Sep 14, 2012·Journal of Insect Science·Ashutosh Sharma, Pratap Kumar Pati
Jan 18, 2013·Journal of Ayurveda and Integrative Medicine·Shweta ShenoyMadan Mohan Paadhi
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Aug 27, 2015·Cellular and Molecular Life Sciences : CMLS·Nawab John DarMuzamil Ahmad
Sep 13, 2015·Neurochemistry International·Renu WadhwaSunil C Kaul
Jul 26, 2015·Molecular Neurobiology·Hardeep KatariaGurcharan Kaur
Apr 22, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Ran GaoRenu Wadhwa
Mar 20, 2015·Biotechnology & Genetic Engineering Reviews·Pritika SinghPratap Kumar Pati
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May 22, 2019·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sukant GargRenu Wadhwa
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Jul 11, 2021·Neurochemistry International·Ashish KumarRenu Wadhwa
Aug 28, 2021·Nutrients·Priyanshu BhargavaSunil C Kaul

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