Selective labeling of sensory hair cells and neurons in auditory, vestibular, and lateral line systems by a monoclonal antibody

The Journal of Comparative Neurology
H I KornblumB Trevarrow

Abstract

This study reports that zn-1, a monoclonal antibody, labels hair cells but not supporting cells in the inner ear and the lateral line of the axolotl salamander, Ambystoma mexicanum. Zn-1 immunocytochemically labels the cytoplasm and stereocilia of mature hair cells in the sacculus, in the utriculus, and in the mechanoreceptive neuromast organs of the lateral line. Lower levels of labeling mark newly formed hair cells in the periphery of the sacculus and in regenerating neuromasts. Zn-1 also selectively labels neuronal processes and perikarya in the lateral line nerves and ganglia and the VIIIth cranial nerve and ganglion. Processes and perikarya are labeled by zn-1 in the dorsolateral medulla oblongata, at sites of termination of the afferent octaval and lateral line neurons. Western blot analysis revealed that zn-1 labels one or more proteins with molecular weights of 80 and 160 kDa. The identity of these protein bands remains to be determined. The presence of a specific epitope expressed in both hair cells and neurons, but not in supporting cells, in the vestibular and auditory epithelia of the ear and in the mechanoreceptive neuromasts of the lateral line suggests shared cytogenetic heritages. These findings are consistent w...Continue Reading

References

Feb 1, 1976·The Journal of Comparative Neurology·P Opdam, R Nieuwenhuys
Mar 1, 1989·The Journal of Comparative Neurology·A Katayama, J T Corwin
Jun 24, 1988·Science·J T Corwin, D A Cotanche
Jan 1, 1986·Hearing Research·L G Tilney, M S Tilney
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J T Corwin
Aug 1, 1966·American Zoologist·W A Van Bergeijk
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
Nov 1, 1984·The Journal of Comparative Neurology·B FritzschU Will
Jan 20, 1981·The Journal of Comparative Neurology·C C Bell
Oct 1, 1981·The Journal of Comparative Neurology·J T Corwin

❮ Previous
Next ❯

Citations

Mar 9, 2013·Experimental & Molecular Medicine·Jeong-Han LeeRaekil Park
Mar 12, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·F J ArenzanaR Arévalo
Oct 1, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Babykumari P ChitramuthuHugh P J Bennett
Jun 29, 2001·Mechanisms of Development·N GompelA Ghysen
Jul 15, 2005·Hearing Research·Christopher Ton, Chuenlei Parng
Feb 28, 2009·Brain Research·Heather R BrignullJennifer S Stone
Feb 28, 2009·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Bahram S DezfuliRoberto Berti
Mar 4, 1998·Current Opinion in Neurology·J S StoneE W Rubel
May 19, 2011·Journal of Applied Toxicology : JAT·Luisa GiariAlessandro Martini
Feb 22, 1994·The Journal of Comparative Neurology·R G NorthcuttB B Criley

❮ Previous
Next ❯

Related Concepts

Related Feeds

© 2021 Meta ULC. All rights reserved