Selective localization of myosin-I proteins in macropinosomes and actin waves

Cytoskeleton
Hanna BrzeskaM A Titus

Abstract

Class I myosins are widely expressed with roles in endocytosis and cell migration in a variety of cell types. Dictyostelium express multiple myosin Is, including three short-tailed (Myo1A, Myo1E, Myo1F) and three long-tailed (Myo1B, Myo1C, Myo1D). Here we report the molecular basis of the specific localizations of short-tailed Myo1A, Myo1E, and Myo1F compared to our previously determined localization of long-tailed Myo1B. Myo1A and Myo1B have common and unique localizations consistent with the various features of their tail region; specifically the BH sites in their tails are required for their association with the plasma membrane and heads are sufficient for relocalization to the front of polarized cells. Myo1A does not localize to actin waves and macropinocytic protrusions, in agreement with the absence of a tail region which is required for these localizations of Myo1B. However, in spite of the overall similarity of their domain structures, the cellular distributions of Myo1E and Myo1F are quite different from Myo1A. Myo1E and Myo1F, but not Myo1A, are associated with macropinocytic cups and actin waves. The localizations of Myo1E and Myo1F in macropinocytic structures and actin waves differ from the localization of Myo1B. M...Continue Reading

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Citations

Sep 28, 2016·Frontiers in Physiology·Kevin D HaBin Liu
Nov 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Alexander JunemannJan Faix
Dec 14, 2016·ELife·Douwe M VeltmanRobert R Kay
Jun 30, 2016·Journal of Cell Science·Gareth Bloomfield, Robert R Kay
Jan 9, 2019·Journal of Cell Science·Thomas D WilliamsRobert R Kay
Mar 2, 2019·Journal of Leukocyte Biology·Daniel Alberto Girón-PérezLeopoldo Santos-Argumedo
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Jason S King, Robert R Kay
Nov 9, 2019·Journal of Experimental & Clinical Cancer Research : CR·Yanhao ZhangNing Gao
Jun 28, 2021·Cells & Development·Robert R Kay
Jan 4, 2022·Cell Adhesion & Migration·Juan D Diaz-ValenciaGenaro Patiño-López

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