Selective medium for isolation of Clostridium botulinum from human feces.

Journal of Clinical Microbiology
M DezfulianV R Dowell

Abstract

A selective medium, Clostridium botulinum isolation (CBI) agar, was developed for the isolation of C. botulinum from human feces. This medium contains cycloserine (250 microgram/ml), sulfamethoxazole (76 microgram/ml), and trimethoprim (4 microgram/ml) as selective inhibitory agents. Qualitative tests indicated complete recovery of C. botulinum types A, B, F, and G on CBI medium. It was more difficult to recognize type G colonies on the medium because of their lack of lipase activity. Except for a few species of Clostridium, the growth of other obligate anaerobes and of the facultative anaerobes tested on CBI medium was suppressed. Quantitative studies of C. botulinum on the selective medium yielded counts comparable to those obtained on egg yolk agar control plates. Isolation of C. botulinum types A, B, and F from seeded fecal specimens was easily achieved with CBI medium. The use of CBI agar should aid the rapid isolation of C. botulinum from fecal specimens associated with foodborne and infant botulism.

References

Jul 1, 1979·Reviews of Infectious Diseases·C L Hatheway
Jul 2, 1977·British Medical Journal·M B Skirrow
Jul 1, 1980·Antimicrobial Agents and Chemotherapy·J M SwensonV R Dowell
Jun 1, 1980·Journal of Clinical Microbiology·C W MossL M McCroskey
Jan 1, 1978·Current Microbiology·J M Swenson, C Thornsberry
Dec 1, 1979·Journal of Food Protection·H M Solomon, D A Kautter

❮ Previous
Next ❯

Citations

Jul 8, 2005·Applied and Environmental Microbiology·Carolina LúquezRafael A Fernández
Apr 1, 1992·Journal of Paediatrics and Child Health·R W ByardP N Goldwater
Oct 1, 1982·European Journal of Clinical Microbiology·N de VosJ Marcelis
Mar 21, 2007·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yoshiaki KasaiTateo Fujii
Dec 10, 1999·Folia Microbiologica·M Dezfulian
Jan 1, 1994·World Journal of Microbiology & Biotechnology·M Dezfulian, J G Bartlett
Nov 20, 2012·Applied and Environmental Microbiology·Daniel LeclairJohn W Austin
Nov 28, 2014·Journal of Clinical Microbiology·Christelle MazuetMichel R Popoff
Apr 15, 2006·Clinical Microbiology Reviews·Miia Lindström, Hannu Korkeala
Aug 8, 2009·Journal of Medical Microbiology·Kathie A GrantJim McLauchlin
Apr 15, 2014·Polish Journal of Veterinary Sciences·T GrendaK Kwiatek
Jan 8, 1983·The Medical Journal of Australia·W G Murrell, B J Stewart
Oct 7, 2017·International Journal of Circumpolar Health·Daniel LeclairJohn W Austin
Oct 1, 1985·Applied and Environmental Microbiology·J C SilasM A Harrison
Dec 1, 1984·Journal of Clinical Microbiology·C Glasby, C L Hatheway
Sep 1, 1984·Journal of Clinical Microbiology·M R Popoff
Jul 1, 1993·Applied and Environmental Microbiology·M C GoodnoughE A Johnson
Sep 21, 2007·Journal of Clinical Microbiology·Ingrid ArtinElisabet Holst
Feb 1, 1985·Journal of Clinical Microbiology·M Dezfulian, J G Bartlett
Aug 1, 1985·Infection and Immunity·M Dezfulian, J G Bartlett
Oct 1, 1991·Applied and Environmental Microbiology·W J Lin, E A Johnson
Feb 21, 2013·Environmental Science & Technology·Chan Lan ChunMichael J Sadowsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.