Selective metal binding by Vanabin2 from the vanadium-rich ascidian, Ascidia sydneiensis samea

Biochimica Et Biophysica Acta
Norifumi KawakamiHitoshi Michibata

Abstract

Vanadium-binding proteins, or Vanabins, have recently been isolated from the vanadium-rich ascidian, Ascidia sydneiensis samea. Recent reports indicate that Vanabin2 binds twenty V(IV) ions at pH 7.5, and that it has a novel bow-shaped conformation. However, the role of Vanabin2 in vanadium accumulation by the ascidian has not yet been determined. In the present study, the effects of acidic pH on selective metal binding to Vanabin2 and on the secondary structure of Vanabin2 were examined. Vanabin2 selectively bound to V(IV), Fe(III), and Cu(II) ions under acidic conditions. In contrast, Co(II), Ni(II), and Zn(II) ions were bound at pH 6.5 but not at pH 4.5. Changes in pH had no detectable effect on the secondary structure of Vanabin2 under acidic conditions, as determined by circular dichroism spectroscopy, and little variation in the dissociation constant for V(IV) ions was observed in the pH range 4.5-7.5, suggesting that the binding state of the ligands is not affected by acidification. Taken together, these results suggest that the reason for metal ion dissociation upon acidification is attributable not to a change in secondary structure but, rather, that it is caused by protonation of the amino acid ligands that complex wi...Continue Reading

References

Apr 17, 2003·Biochimica Et Biophysica Acta·Tatsuya UekiHitoshi Michibata
Oct 8, 1962·Biochimica Et Biophysica Acta·J P HUMMEL, W J DREYER
Mar 27, 2004·Biochemical and Biophysical Research Communications·Piyali Guha ThakurtaJ K Dattagupta
Aug 9, 2005·Biochimica Et Biophysica Acta·Masao YoshiharaHitoshi Michibata

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Citations

Dec 19, 2006·Trends in Biotechnology·Scott GiffordGeoff R MacFarlane
Apr 22, 2015·Environment International·Muhammad ImtiazShuxin Tu
Aug 5, 2016·Environmental Science and Pollution Research International·Evren TuncaÜlküAlver Şahin
Mar 11, 2018·Biological Trace Element Research·Deepika TripathiRavi Prakash Pal
May 1, 2010·Biomolecular Concepts·Hitoshi Michibata, Tatsuya Ueki
Mar 17, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Jean P Gaffney, Ann M Valentine
May 11, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Hiroki KitayamaTatsuya Ueki

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