Selective modulation of MAP kinase in embryonic palate cells

Journal of Cellular Physiology
M B PotchinskyR M Greene

Abstract

Murine embryonic palate mesenchyme (MEPM) cells are responsive to a number of endogenous factors found in the local embryonic tissue environment. Recently, it was shown that activation of the cyclic AMP (cAMP) or the transforming growth factor beta (TGFbeta) signal transduction pathways modulates the proliferative response of MEPM cells to epidermal growth factor (EGF). Since the mitogen-activated protein kinase (MAPK) cascade is a signal transduction pathway that mediates cellular responsiveness to EGF, we examined the possibility that several signaling pathways which abrogate EGF-stimulated proliferation do so via the p42/p44 MAPK signaling pathway. We demonstrate that EGF stimulates MAPK phosphorylation and activity in MEPM cells maximally at 5 minutes. Tyrosine phosphorylation and activation of MAPK was unaffected by treatment of MEPM cells with TGFbeta or cholera toxin. Similarly, TGFbeta altered neither EGF-induced MAPK tyrosine phosphorylation nor activity. However, the calcium ionophore, A23187, significantly increased MAPK phosphorylation which was further increased in the presence of EGF, although calcium mobilization reduced EGF-induced proliferation. Despite the increase in phosphorylation, we could not demonstrate ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1983·Experimental Cell Research·R I Grove, R M Pratt
Nov 17, 1995·The Journal of Biological Chemistry·K TeradaT Satoh
Mar 8, 1995·Biochemical and Biophysical Research Communications·V MitevL M Houdebine
Oct 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M S Marshall
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·D T DudleyA R Saltiel
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B R SevetsonJ C Lawrence
Nov 12, 1993·Science·S J Cook, F McCormick
Mar 31, 1995·The Journal of Biological Chemistry·M T Hartsough, K M Mulder
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·J S CampbellE G Krebs
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R FabianD K Morrison
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Blenis
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·L B Rosen, M E Greenberg
Sep 1, 1996·Molecular Biology of the Cell·R E HerreraR A Weinberg

❮ Previous
Next ❯

Citations

Jun 22, 2000·Teratology·R M Greene, M M Pisano
Aug 1, 2000·Brain Research. Developmental Brain Research·R D LearishM Haak-Frendscho
Jun 17, 2005·Human & Experimental Toxicology·Chada S Reddy
Jul 26, 2003·In Vitro Cellular & Developmental Biology. Animal·Vasker BhattacherjeeM Michele Pisano
Dec 21, 2006·Birth Defects Research. Part A, Clinical and Molecular Teratology·Saurabh SinghRobert M Greene
Sep 10, 2009·Birth Defects Research. Part A, Clinical and Molecular Teratology·Saurabh SinghM Michele Pisano
Aug 28, 1999·The Journal of Biological Chemistry·L L DuganD H Gutmann
Apr 20, 2002·In Vitro Cellular & Developmental Biology. Animal·D R WarnerR M Greene
Dec 4, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayM Michele Pisano
Oct 10, 2019·Journal of Applied Oral Science : Revista FOB·Xuan ShuShenyou Shu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.