Selective Monitoring and Imaging of Eosinophil Peroxidase Activity with a J-Aggregating Probe

Journal of the American Chemical Society
Tae-Il KimYoungmi Kim

Abstract

The specific detection of eosinophil peroxidase (EPO) activity requires the difficult distinction between hypobromous acid generated by EPO and hypochlorous acid generated by other haloperoxidases. Here we report a fluorogenic probe that is halogenated with high kinetic selectivity (≥1200:1) for HOBr over HOCl. Heavy-atom effects do not quench the dibrominated product because of its self-assembly into emissive J-aggregates that provide a turn-on signal. Applications of this fluorogen to EPO activity assays, dipstick sensors, fluorescence imaging of EPO activity, assays of oxidative stress in cancer cells, and immune response detection in live mice are reported.

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Citations

Nov 28, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Maria StrianeseClaudio Pellecchia
Jun 1, 2019·Chemical Communications : Chem Comm·Jian-Xin WangRoman Boulatov
May 14, 2020·The Analyst·David HartnellMark J Hackett
Nov 12, 2019·Frontiers in Chemistry·Zhipeng LiuXiaoqing Wang
Apr 25, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chenwen ShaoYong Qian
Jan 16, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuyu Fang, Wim Dehaen
Apr 24, 2021·Nature Communications·Kang LiZhipeng Liu

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