PMID: 8591066Oct 1, 1995Paper

Selective permeability of [3H]-D-mannitol and [14C]-carboxyl-inulin across the blood-brain barrier and blood-spinal cord barrier in the rabbit

The Journal of Spinal Cord Medicine
Leon D ProckopJ Ransohoff

Abstract

Selective permeability across the blood-brain and blood-spinal cord barriers was studied in a rabbit experimental model. To maintain steady state levels of the tracers in the circulation, 50muCi each of [3H]-D-mannitol and [14C]-carboxyl-inulin were administered as a bolus and by slow intravenous infusion for 60, 90 and 120 minutes; 90 min proved to be the optimal equilibration time for uptake of radioactive tracers into central nervous system (CNS) regions. Kinetic transfer of [3H]-D-mannitol and [14C]-carboxyl-inulin from blood into CNS was computed as apparent transfer constant (KD). The KD for [3H]-D-mannitol in regions of brain (cerebrum, cerebellum, mid-brain, pons and brain stem), and spinal cord (cervical, thoracic and lumbar) were 0.033 to 0.054 microliter/min/g-1 and 0.053 to 0.065 microliter/min/g-1, respectively. The KD for [14C]-carboxyl-inulin into brain and spinal cord regions were 0.016 to 0.033 and 0.037 to 0.054 microliter/min/g-1, respectively. Of the regions of spinal cord and brain examined, lumbar cord appears to be the most permeable. The KD values pooled for samples of the spinal cord show a significant increase in uptake of [3H]-D-mannitol (p = 0.0043) and [14C]-carboxyl-inulin (p = 0.0001) compared to ...Continue Reading

References

Mar 1, 1969·The Journal of Cell Biology·M W Brightman, T S Reese

❮ Previous
Next ❯

Citations

Jul 12, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P W StromanB Tomanek
Oct 24, 2014·Frontiers in Cellular Neuroscience·Marcos F DosSantosVivaldo Moura-Neto
Jul 2, 2018·Fluids and Barriers of the CNS·Servio H RamirezJoel S Pachter
Dec 14, 2016·Pflügers Archiv : European journal of physiology·A K Reinhold, H L Rittner
Aug 6, 2019·Neuroscience Journal·Nicholas KakaroubasNitin K Saksena
Jun 21, 2012·Molecular Pain·Dimitris N XanthosJürgen Sandkühler
Jun 16, 2011·Annals of Neurology·Viktor BartanuszMurat Digicaylioglu
Jun 13, 2012·Brain : a Journal of Neurology·Véronique SchaefferMichel Goedert
Aug 2, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ethan A WinklerBerislav V Zlokovic
Jul 11, 2013·Mediators of Inflammation·Beatrice Mihaela RaduPaolo Francesco Fabene
May 4, 2016·Tissue Barriers·Imola WilhelmIstván A Krizbai
Dec 10, 2020·Journal of Neurotrauma·Lin-Yu JinHai-Ying Liu
Apr 2, 2021·Biological & Pharmaceutical Bulletin·Yasuo Uchida

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.