Abstract
Selective permeability across the blood-brain and blood-spinal cord barriers was studied in a rabbit experimental model. To maintain steady state levels of the tracers in the circulation, 50muCi each of [3H]-D-mannitol and [14C]-carboxyl-inulin were administered as a bolus and by slow intravenous infusion for 60, 90 and 120 minutes; 90 min proved to be the optimal equilibration time for uptake of radioactive tracers into central nervous system (CNS) regions. Kinetic transfer of [3H]-D-mannitol and [14C]-carboxyl-inulin from blood into CNS was computed as apparent transfer constant (KD). The KD for [3H]-D-mannitol in regions of brain (cerebrum, cerebellum, mid-brain, pons and brain stem), and spinal cord (cervical, thoracic and lumbar) were 0.033 to 0.054 microliter/min/g-1 and 0.053 to 0.065 microliter/min/g-1, respectively. The KD for [14C]-carboxyl-inulin into brain and spinal cord regions were 0.016 to 0.033 and 0.037 to 0.054 microliter/min/g-1, respectively. Of the regions of spinal cord and brain examined, lumbar cord appears to be the most permeable. The KD values pooled for samples of the spinal cord show a significant increase in uptake of [3H]-D-mannitol (p = 0.0043) and [14C]-carboxyl-inulin (p = 0.0001) compared to ...Continue Reading
Citations
Jul 12, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P W StromanB Tomanek
Jun 19, 2013·BMC Neuroscience·P Marc D WatsonCarl I Webster
Oct 24, 2014·Frontiers in Cellular Neuroscience·Marcos F DosSantosVivaldo Moura-Neto
Jul 2, 2018·Fluids and Barriers of the CNS·Servio H RamirezJoel S Pachter
Jul 10, 2018·Molecular Neurobiology·Yasuo UchidaTetsuya Terasaki
Nov 12, 2016·Journal of Neuroinflammation·Yves MolinoMichel Khrestchatisky
Dec 14, 2016·Pflügers Archiv : European journal of physiology·A K Reinhold, H L Rittner
Aug 6, 2019·Neuroscience Journal·Nicholas KakaroubasNitin K Saksena
Aug 1, 1997·Anesthesia and Analgesia·E S FuL D Prockop
Jun 21, 2012·Molecular Pain·Dimitris N XanthosJürgen Sandkühler
Jun 16, 2011·Annals of Neurology·Viktor BartanuszMurat Digicaylioglu
Jun 13, 2012·Brain : a Journal of Neurology·Véronique SchaefferMichel Goedert
Jan 5, 2002·Anesthesia and Analgesia·Kamatham A NaiduLeon D Prockop
Aug 2, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ethan A WinklerBerislav V Zlokovic
Jul 11, 2013·Mediators of Inflammation·Beatrice Mihaela RaduPaolo Francesco Fabene
May 4, 2016·Tissue Barriers·Imola WilhelmIstván A Krizbai
Oct 12, 2017·Scientific Reports·Magdalena A LamMarcus A Stoodley
Dec 10, 2020·Journal of Neurotrauma·Lin-Yu JinHai-Ying Liu
Sep 24, 2018·Experimental Neurology·Yang D TengRoss D Zafonte
Apr 2, 2021·Biological & Pharmaceutical Bulletin·Yasuo Uchida
Aug 1, 1997·Experimental Neurology·W PanA J Kastin
May 14, 2021·Pain Reports·Karli Montague-Cardoso, Marzia Malcangio