Selective precipitation of proteins

Current Protocols in Protein Science
R E Lovrien, D Matulis

Abstract

Selective precipitation of proteins can be used as a bulk method to recover the majority of proteins from a crude lysate, as a selective method to fractionate a subset of proteins from a protein solution, or as a very specific method to recover a single protein of interest from a purification step. This unit describes a number of methods suitable for selective precipitation. In each of the protocols that are outlined, the physical or chemical basis of the precipitation process, the parameters that can be varied for optimization, and the basic steps for developing an optimized precipitation are described.

References

Dec 1, 1978·Archives of Biochemistry and Biophysics·S I Miekka, K C Ingham
Aug 1, 1977·Nucleic Acids Research·T A BickleR Imber
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Dec 1, 1988·Journal of Biochemical and Biophysical Methods·T N CollingwoodA G Langdon
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Mar 14, 1974·Biochimica Et Biophysica Acta·M Sternberg, D Hershberger
Jan 1, 1970·Biotechnology and Bioengineering·M Z Sternberg
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·A L Fink
Nov 20, 1993·Journal of Molecular Biology·P Chakrabarti
Sep 1, 1996·Journal of Molecular Recognition : JMR·D MatulisT I Richardson
Apr 1, 1953·Archives of Biochemistry and Biophysics·D R WHITAKER
Jan 1, 1956·Advances in Protein Chemistry·F R GURD, P E WILCOX
Jan 1, 1960·Advances in Protein Chemistry·K FELIX
Jan 1, 1959·Advances in Protein Chemistry·M SELA, E KATCHALSKI
Nov 5, 1994·Biotechnology and Bioengineering·C S PatrickiosT A Hatton
Jan 10, 1996·Accounts of Chemical Research·Judith Herzfeld

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Citations

Apr 3, 2016·Current Protocols in Protein Science

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