Selective Pressure of Temperature on Competition and Cross-Feeding within Denitrifying and Fermentative Microbial Communities

Frontiers in Microbiology
Anna HankeMarc Strous

Abstract

In coastal marine sediments, denitrification and fermentation are important processes in the anaerobic decomposition of organic matter. Microbial communities performing these two processes were enriched from tidal marine sediments in replicated, long term chemostat incubations at 10 and 25°C. Whereas denitrification rates at 25°C were more or less stable over time, at 10°C denitrification activity was unstable and could only be sustained either by repeatedly increasing the amount of carbon substrates provided or by repeatedly decreasing the dilution rate. Metagenomic and transcriptomic sequencing was performed at different time points and provisional whole genome sequences (WGS) and gene activities of abundant populations were compared across incubations. These analyses suggested that a temperature of 10°C selected for populations related to Vibrionales/Photobacterium that contributed to both fermentation (via pyruvate/formate lyase) and nitrous oxide reduction. At 25°C, denitrifying populations affiliated with Rhodobacteraceae were more abundant. The latter performed complete denitrification, and may have used carbon substrates produced by fermentative populations (cross-feeding). Overall, our results suggest that a mixture of...Continue Reading

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Citations

Jul 12, 2020·Environment International·Tangran HuoSitong Liu
Oct 9, 2021·Frontiers in Microbiology·Flavia TarquinioAndrew Bissett

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Datasets Mentioned

BETA
PRJNA255460

Methods Mentioned

BETA
PCR
RNA-Seq
phylogenetic profiles
amplicon sequencing

Software Mentioned

MetaWatt
FastTree2
BLASTx
bowtie2
EMIRGE
Torrent Suite
GS De Novo assembler
MAFFT

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