Selective probing and imaging of cells with single walled carbon nanotubes as near-infrared fluorescent molecules

Nano Letters
Kevin WelsherHongjie Dai

Abstract

Fluorescent molecules emitting in the near-infrared (NIR, wavelength approximately 0.8-2 microm) are relatively scarce and have been actively sought for biological applications because cells and tissues exhibit little auto-fluorescence in this region. Here, we report the use of semiconducting single-walled carbon nanotubes (SWNTs) as near-infrared fluorescent tags for selective probing of cell surface receptors and cell imaging. Biologically inert SWNTs with polyethyleneglycol functionalization are conjugated to antibodies such as Rituxan to selectively recognize CD20 cell surface receptor on B-cells with little nonspecific binding to negative T-cells and Herceptin to recognize HER2/neu positive breast cancer cells. We image selective SWNT-antibody binding to cells by detecting the intrinsic NIR photoluminescence of nanotubes. We observe ultralow NIR autofluorescence for various cells, an advantageous feature over high autofluorescence and large variations between cells lines in the visible. This establishes SWNTs as novel NIR fluorophors for sensitive and selective biological detections and imaging in vitro and potentially in vivo. Further, our results clearly show that the interactions between carbon nanotubes and living cell...Continue Reading

References

Jul 27, 2002·Science·Michael J O'ConnellRichard E Smalley
Dec 3, 2002·Science·Sergei M BachiloR Bruce Weisman
Dec 2, 2004·Journal of the American Chemical Society·Paul CherukuriR Bruce Weisman
Feb 9, 2005·Physical Review Letters·Vasili PerebeinosPhaedon Avouris
Dec 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paul CherukuriR Bruce Weisman
Jun 8, 2007·Journal of the American Chemical Society·Jared CrochetTobias Hertel
Jun 16, 2007·Journal of the American Chemical Society·Rodney P FeazellStephen J Lippard
Sep 21, 2007·Nano Letters·Dmitri A TsyboulskiR Bruce Weisman
Nov 14, 2007·Nano Letters·Lisa J CarlsonTodd D Krauss

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Citations

Nov 10, 2009·Analytical and Bioanalytical Chemistry·Zhi ZhuWeihong Tan
Aug 14, 2010·Analytical and Bioanalytical Chemistry·Emer LahiffDermot Diamond
May 13, 2010·European Journal of Nuclear Medicine and Molecular Imaging·Zhuang Liu, Rui Peng
Dec 3, 2011·Annals of Biomedical Engineering·Joseph K YoungRebekah A Drezek
May 7, 2013·Colloids and Surfaces. B, Biointerfaces·Hongying MaoMorteza Mahmoudi
Feb 23, 2013·ACS Applied Materials & Interfaces·Elena HeisterAlan B Dalton
Aug 10, 2013·ACS Applied Materials & Interfaces·Yoko IizumiMasako Yudasaka
Jan 5, 2014·Environmental Science & Technology·Joseph H BisesiTara Sabo-Attwood
Sep 23, 2008·Journal of the American Chemical Society·Zhuang LiuHongjie Dai
Dec 5, 2008·Journal of the American Chemical Society·Dmitri A TsyboulskiR Bruce Weisman
Dec 9, 2008·Journal of the American Chemical Society·Andrew P GoodwinHongjie Dai
Jan 29, 2009·Journal of the American Chemical Society·Giuseppe PrencipeHongjie Dai
Mar 2, 2012·Nano Letters·Santanu BhattacharyaAnand Jagota
Feb 1, 2012·ACS Nano·Christophe M LamyJean-Yves Chatton
Nov 26, 2009·ACS Nano·David K BwambokIsiah M Warner
Oct 28, 2008·Nature Biotechnology·Zhuo ChenHongjie Dai
Nov 7, 2009·Nature Nanotechnology·Kevin WelsherHongjie Dai
Sep 5, 2009·Nature Protocols·Zhuang LiuHongjie Dai
Oct 29, 2008·Chemical Communications : Chem Comm·Qiao-Ling ZhaoDai-Wen Pang
Jun 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Pavitra ChakravartyEllen S Vitetta
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kevin WelsherHongjie Dai
Jul 7, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Sofia I PascuZhiyuan Hu
Aug 15, 2008·Cancer Research·Zhuang LiuHongjie Dai
Mar 5, 2009·Molecular Cancer Therapeutics·Ji Yuan WuRazelle Kurzrock
Apr 19, 2011·International Journal of Nanomedicine·Achraf Al FarajEmmanuelle Canet-Soulas
Mar 15, 2011·Immunotherapy·Francois Fay, Christopher J Scott
Nov 1, 2010·Therapeutic Delivery·Himanshu BhattacharjeeGeorge C Wood
Nov 3, 2010·Nanomedicine·Vivek S ThakareSanyog Jain
Nov 5, 2013·Advanced Drug Delivery Reviews·Hua GongZhuang Liu
Aug 13, 2013·Advanced Drug Delivery Reviews·Ravi Singh, Suzy V Torti
Apr 3, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Renping CaoJianrong Qiu
Nov 12, 2013·Biotechnology Advances·Yu GaoLee Jia
Apr 29, 2014·Journal of Nanobiotechnology·Azamal Husen, Khwaja Salahuddin Siddiqi

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