Selective receptor blockade during phagocytosis does not alter the survival and growth of Mycobacterium tuberculosis in human macrophages

American Journal of Respiratory Cell and Molecular Biology
S ZimmerliJ D Ernst

Abstract

Mycobacterium tuberculosis survives and replicates within human macrophages, but the mechanisms whereby tubercle bacilli resist killing are incompletely understood. We tested the general model in which M. tuberculosis evades killing by entering naive macrophages through receptors that are unable to activate cellular microbicidal activities. Complement receptor types 1 (CR1), 3 (CR3), and 4 (CR4) were blocked with monoclonal antibodies, and mannose receptors were blocked with a competitive ligand, mannosylated bovine serum albumin (MBSA). Survival and replication of M. tuberculosis (Erdman) were evaluated after the bacteria were phagocytosed in the presence of blocking agents (directing binding to the unblocked receptors). Although there was significant variation in the growth rate of virulent M. tuberculosis in monocyte-derived macrophages from different donors, the intracellular survival and replication of mycobacteria were equivalent regardless of the receptor(s) used for binding and phagocytosis. We conclude that the mechanisms whereby M. tuberculosis evades killing by human macrophages are independent of the receptor-mediated route of entry, and operate at one or more steps common to all entry pathways. Blocking complement ...Continue Reading

Citations

Aug 27, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Rogelio Hernandez-PandoDiana Aguilar
Aug 21, 2003·Microbial Pathogenesis·Marco Antonio Velasco-VelázquezJosé Agramonte-Hevia
Jan 13, 2001·Current Opinion in Immunology·J Pieters
Apr 2, 1998·Current Opinion in Immunology·P D Stahl, R A Ezekowitz
Feb 28, 2002·Trends in Microbiology·Jean Pieters, John Gatfield
Apr 12, 2000·Microbes and Infection·P J Gough, S Gordon
Feb 28, 2001·Microbes and Infection·L E Bermudez, F J Sangari
May 19, 2001·Microbes and Infection·J Pieters
Dec 28, 1999·Microbes and Infection·G R Strohmeier, M J Fenton
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·D G Russell
Jan 6, 2000·The Journal of Experimental Medicine·C A ThomasJ El Khoury
Apr 5, 2002·Clinical Microbiology Reviews·Reinout van CrevelJos W M van der Meer
Jul 15, 2003·Clinical Microbiology Reviews·Edwin S Van AmersfoortJohan Kuiper
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Kassidy K Huynh, Sergio Grinstein
Jul 16, 2011·International Journal of Cell Biology·Luisa Jordao, Otilia V Vieira
Jul 20, 2012·Clinical & Developmental Immunology·Mayra Silva MirandaFrederic Altare
Mar 28, 1998·American Journal of Respiratory and Critical Care Medicine·N W Schluger, W N Rom
Jun 25, 1998·American Journal of Respiratory and Critical Care Medicine·C AstonJ Reibman
Dec 22, 2006·PloS One·Olivier NeyrollesBrigitte Gicquel
Jan 6, 2010·EMBO Molecular Medicine·Alessandra MortellaroPaola Ricciardi-Castagnoli
Nov 8, 2011·Annual Review of Pathology·Jennifer A Philips, Joel D Ernst
Jan 15, 2011·Developmental and Comparative Immunology·Stephanie WiddisonTracey J Coffey
Dec 17, 2009·Tuberculosis·Zissis C ChroneosChinnaswamy Jagannath
Jun 10, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yolandy LemmerChantal de Chastellier
Jun 6, 2015·Cellular Microbiology·Victoria CanoJosé A Bengoechea
Sep 19, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Szczepan JózefowskiKatarzyna Kwiatkowska
Jul 17, 2010·Cellular Microbiology·Tyler B M HickeyRichard W Stokes
Sep 10, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Hsin-Tsung HoYen-Ta Lu

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