Selective regulation of class I and class II histone deacetylases expression by inhibitors of histone deacetylases in cultured mouse neural cells

Neuroscience Letters
Farzam AjamianMati Reeben

Abstract

The expression of 10 histone deacetylases (HDAC1-10) mRNAs in mouse neuroblastoma Neuro-2a and microglia N9 cell cultures after treatment by inhibitors of HDACs, sodium butyrate and trichostatin A was studied to elucidate whether HDAC inhibitors affect the gene expression of HDACs themselves. Northern blot analysis demonstrated two- to four-fold elevated levels of mRNAs for HDAC1, -3, -5, and -6 after drug treatment in comparison with untreated cells, while mRNA levels for HDAC2 and -7 did not change significantly. In both Neuro-2a and N9 cells the highest increase was observed for HDAC5 and -6 mRNA, whereas, HDAC4 had a prominent increase in mRNA levels after drug treatment only in N9 microglia cell line but not in Neuro-2a. Immunocytochemical examination confirmed that changes in protein levels of HDACs were similar to changes in their mRNA levels. There exists an auto-regulatory feedback loop to the expression levels of several HDACs after inhibition of their biochemical activity, adding several HDAC genes to the list of genes regulated by HDAC inhibitors.

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Citations

May 25, 2011·World Journal of Stem Cells·Naiara Z SaraivaJoaquim M Garcia
Mar 26, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ambalika SarkarVidita A Vaidya
Feb 5, 2016·The Journal of Pain : Official Journal of the American Pain Society·Katsuya KamiEmiko Senba
Nov 30, 2005·Journal of Neurochemistry·Lyda M Rincón CastroLennard P Niles
Sep 1, 2007·International Journal of Urology : Official Journal of the Japanese Urological Association·Dexuan GaoHui Zhang
Dec 18, 2004·Toxicology Letters·Shigehiro OsadaTsutomu Nishihara
Feb 19, 2013·Neuroscience Letters·Lennard P NilesAyush Lacoul
Aug 2, 2018·Clinical Epigenetics·Aline KaletschGünter Niegisch
Apr 25, 2007·Nature Reviews. Neuroscience·Nadia TsankovaEric J Nestler
Jan 23, 2018·Journal of Neurochemistry·Elizabeth A Thomas, Santosh R D'Mello
Feb 14, 2008·Medicinal Research Reviews·Yujun George ZhengMasha Goodman
Dec 16, 2019·Neurochemistry International·Mario J BertogliatRaghu Vemuganti

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