PMID: 7017720Jan 1, 1981Paper

Selective removal of apolipoprotein B-containing serum lipoproteins from blood plasma

Proceedings of the National Academy of Sciences of the United States of America
W Stoffel, T Demant

Abstract

Studies were undertaken to determine the applicability and effectiveness of immunoadsorption chromatography on anti-low density lipoprotein (LDL) columns as a plasma-cholesterol-lowering procedure. Mass production and isolation of monospecific antibodies against swine LDL (rho = 1.006-1.063 g/ml) was carried out by immunization of sheep with swine LDL and selective antibody adsorption from their antisera by chromatography on LDL-Sepharose. The isolated LDL antibodies were then covalently linked to Sepharose CL-4B. Pig plasma LDL was effectively removed in vitro by the anti-LDL-Sepharose beads. In vivo studies were performed in pigs with (i) a plasma-separator membrane permeable to solutes below Mr 2,000,000 or (ii) a blood centrifuge interposed into an arteriovenous shunt to separate the corpuscular elements of blood from the plasma. In either case, the plasma was passed through the anti-LDL-Sepharose column and recirculated into the venous part of the shunt. Plasma cholesterol levels were reduced 70-80% by this procedure and rebounded 3-4 days after the extracorporeal immunoadsorption procedure. This continuous plasma separation-immunoadsorption procedure may have broad applicability for the elimination of any plasma component...Continue Reading

References

Jun 17, 1976·The New England Journal of Medicine·M S Brown, J L Goldstein
Oct 1, 1978·Canadian Journal of Biochemistry·Y L MarcelD Jeffries
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C PittmanD Steinberg
Feb 15, 1979·FEBS Letters·T KuusiE A Nikkilä
Sep 20, 1977·Biochemistry·K FujikawaE W Davie
Nov 4, 1975·Biochemistry·W Kisiel, E W Davie
Sep 1, 1975·Thrombosis Research·L O AnderssonM Miller-Andersson
Jun 1, 1972·The Journal of Clinical Investigation·T LangerR I Levy
Nov 20, 1973·Biochemistry·K FujikawaE W Davie
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Aug 1, 1966·Canadian Journal of Biochemistry·M JanadoW H Cook
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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Citations

Jan 1, 1995·Journal of Molecular Recognition : JMR·N UbrichC Rivat
Dec 15, 1988·Klinische Wochenschrift·I SchenckN Zöllner
Jan 1, 1984·Applied Biochemistry and Biotechnology·S SidemanA Tzipiniuk
Apr 7, 2007·Bulletin of Experimental Biology and Medicine·O I Afanas'evaS N Pokrovskii
May 13, 2008·Journal of Materials Science. Materials in Medicine·Kai-Wang MaWan-Jun Lei
Dec 14, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·D GairinJ C Fruchart
Sep 30, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·D ErlichH Weintraub
Sep 1, 1984·Biomaterials·T UchidaY Murao
Jun 23, 1995·Journal of Chromatography. B, Biomedical Applications·T AdachiK Kojima
Jun 6, 1981·Lancet·G R Thompson
May 11, 1985·Lancet·V V TertovS N Pokrovsky
Apr 25, 2003·Biomaterials·Shenqi WangYan Cheng
Jan 6, 2000·Transfusion Science·H Borberg
May 15, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adil Denizli
May 11, 2000·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·N Tani
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A MarciniakE Haber
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T S ParkerE H Ahrens
Apr 1, 1991·European Journal of Clinical Investigation·G FranceschiniC R Sirtori
Dec 9, 2000·Transplant International : Official Journal of the European Society for Organ Transplantation·P BrennerB Reichart
Aug 1, 1997·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·A YamamotoS Kojima
Jun 17, 2003·Journal of Biomaterials Science. Polymer Edition·Handan Yavuz, Adil Denizli
Jan 8, 2014·Journal of Materials Science. Materials in Medicine·Jinghua LiKaiyong Cai
Aug 4, 1995·Journal of Chromatography. B, Biomedical Applications·A Denizli, E Pişkin

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Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.