Selective retinol production by modulating the composition of retinoids from metabolically engineered E. coli

Biotechnology and Bioengineering
Hui-Jeong JangSeon-Won Kim

Abstract

Retinoids can be produced from E. coli when introduced with the β-carotene biosynthesis pathway and the BCMO gene. E. coli has no inherent metabolic pathways related to retinoids, therefore only retinal should be produced from the cleavage of β-carotene by BCMO. However, retinol and retinyl acetate were also produced in significant amounts, by the non-specific activity of inherent promiscuous enzymes or the antibiotic resistance marker of the retinal-producing plasmids. Retinol was produced by the ybbO gene of E. coli which encodes oxidoreductase and retinyl acetate was produced by the chloramphenicol resistance gene, called cat gene which encodes chloramphenicol acetyltransferase, present within the pS-NA plasmid that also contains the mevalonate pathway. The composition of retinoids could be modulated by manipulating the relevant genes. The composition of retinol, a commercially important retinoid, was significantly increased by the overexpression of ybbO gene and the removal of cat gene in the recombinant E. coli, which suggests the possibility of selective retinoid production in the future.

References

Oct 1, 1966·The Journal of Clinical Investigation·D S GoodmanT Shiratori
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A C Ross
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Feb 19, 2002·Chemical Reviews·Lubomír Lapcík L Jr andPeter Chabrecek
Apr 16, 2002·Nature Genetics·Thomas Perlmann
May 12, 2006·Journal of Neurobiology·Rune Blomhoff, Heidi Kiil Blomhoff
Oct 18, 2008·Chemico-biological Interactions·Seung-Ah LeeNatalia Y Kedishvili
Jan 1, 2009·American Journal of Physiology. Endocrinology and Metabolism·Yuguang Shi, Dong Cheng
Dec 30, 2009·Human Genomics·Enrico GarattiniMineko Terao
Feb 5, 2011·Chemico-biological Interactions·Jan-Olov Höög, Linus J Ostberg
Jun 4, 2013·Metabolic Engineering·Jorge Alonso-GutierrezTaek Soon Lee
Mar 13, 2014·Nature Chemical Biology·Gabriel M RodriguezShota Atsumi
Aug 12, 2014·Metabolic Engineering·Gabriel M Rodriguez, Shota Atsumi

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Citations

Aug 2, 2015·Applied Microbiology and Biotechnology·Seung-Hye HongDeok-Kun Oh
Oct 28, 2017·Biotechnology and Bioengineering·Congqiang ZhangHeng-Phon Too
Apr 24, 2016·Applied and Environmental Microbiology·Seung-Hye HongDeok-Kun Oh
Oct 14, 2018·Applied and Environmental Microbiology·Jeffrey R Dwulit-SmithKatrina T Forest
Mar 20, 2019·Microbial Cell Factories·Chonglong WangSeon-Won Kim
Feb 6, 2021·Critical Reviews in Biotechnology·Ming-Hua LiangJian-Guo Jiang
Nov 19, 2020·Antioxidants·Bo Hyun ChoiPyung Cheon Lee
Jan 28, 2020·Current Opinion in Biotechnology·Jong-Won Lee, Cong T Trinh
Jul 8, 2021·Natural Product Reports·Mauro A RinaldiNigel S Scrutton

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