PMID: 7536790Apr 1, 1995Paper

Selective sensitivity of macrophages to cytotoxicity by inhibitors of macromolecular synthesis: induction of apoptosis

Journal of Leukocyte Biology
J G LewisS Fan

Abstract

Initial studies designed to measure the effect of inhibiting RNA synthesis by dactinomycin on macrophage functions revealed that the cells were uniformly killed at concentrations that have been routinely used to inhibit RNA synthesis in other cell types. We, thus, determined the dose curve for the cytotoxicity of dactinomycin for macrophages and two other cell types, L929 cells and splenic lymphocytes. Macrophages were extremely sensitive to the cytotoxicity of dactinomycin compared to the other cell types. Submicromolar concentrations that induced 100% cytotoxicity in macrophages caused little death in L929 cells or lymphocytes. Concentrations of dactinomycin that inhibited RNA synthesis by 40% in macrophages induced almost complete cell death but inhibition of over 80% of RNA synthesis in L929 cells or lymphocytes induced no measurable cytotoxicity. Macrophages did take up more dactinomycin than other cells but the amount was not sufficient to account for the large differences in cytotoxicity. We next tested the effects of doxorubicin and cycloheximide and found that macrophages were also extremely sensitive to killing by these compounds, and there was a very close association between the amount of inhibition of protein synth...Continue Reading

Citations

Jun 5, 2003·Journal of Lipid Research·Peter Gargalovic, Ladislav Dory
Aug 24, 1999·Journal of Cellular Physiology·C E Scott, F Adebodun
Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K FettucciariP Marconi
Aug 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierD Subtil
Mar 1, 2003·International Journal of Immunopharmacology·C D MorganJ W Measel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2021 Meta ULC. All rights reserved