Selective sequestration of cytokine-producing monocytes during hemodialysis treatment

American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation
M GirndtH Köhler

Abstract

Hemodialysis treatment leads to leukocyte activation and cytokine production. Studying this effect has been complicated because cell activation by blood membrane contact also induces adherence factors on leukocytes, leading to margination of cells to the endothelium of the lung. Using single-cell cytokine determination, we studied the relation between cytokine production and cell sequestration during dialysis therapy. Blood was sampled in 11 chronic hemodialysis patients using hemophane dialyzers before hemodialysis and at 20 and 120 minutes of treatment. Lipopolysaccharide (LPS)-induced cytokine production in monocytes was studied by intracellular staining for interleukin-6 (IL-6) and IL-10 and flow cytometry. Results obtained in dialysis patients were compared with samples from an ex vivo dialysis system. Monocyte maturation stage was evaluated by detection of several surface markers through flow cytometry. Within 20 minutes of hemodialysis, the numbers of circulating monocytes decreased to one third of initial values. Before dialysis, 56.7% +/- 15.7% of circulating monocytes responded to LPS by the production of IL-6. This fraction decreased to 21.1% +/- 17.3% (P < 0.001 versus before hemodialysis) at 20 minutes and 32.3% +/...Continue Reading

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Citations

Dec 31, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Alejandro Martin-MaloPedro Aljama
Nov 15, 2002·Radiographics : a Review Publication of the Radiological Society of North America, Inc·Charito LoveChristopher J Palestro
Dec 30, 2004·Cytometry. Part B, Clinical Cytometry·Bruce H Davis, Petar V Zarev
Mar 29, 2008·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·P BurraA K Burroughs
Jun 28, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·U SesterH Köhler
May 28, 2020·Toxins·Matthias GirndtChristof Ulrich
Oct 21, 2020·Jornal brasileiro de nefrologia : ʹorgão oficial de Sociedades Brasileira e Latino-Americana de Nefrologia·Nordeval Cavalcante Araújo, José Hermógenes Rocco Suassuna

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