Selective suppression of follicle-stimulating hormone secretion in anterior pituitary cells by the gonadal steroid 3 alpha-hydroxy-4-pregnen-20-one

Endocrinology
P H Wood, J P Wiebe

Abstract

The effect of 3 alpha-hydroxy-4-pregnen-20-one (3HP), a Sertoli cell steroid, on the secretion of gonadotropins from rat anterior pituitary cells in culture was examined and subsequently compared with the action of other gonadal steroids and steroids structurally related to 3HP. Pituitary cells from randomly cycling, sexually mature female rats were isolated and maintained in culture 72 h before use. On the day of treatment, medium was changed, steroids (10(-16)-10(-4) M) and/or LHRH (10(-8) M) were added, and cells were allowed to incubate for a further 24 h. Medium was then examined for gonadotropin content by RIA. 3HP treatment of anterior pituitary cells resulted in a significant reduction of both basal and LHRH-induced FSH secretion, while LH secretion was unaffected. The lowest effective dose of 3HP (10(-16) M) significantly decreased basal FSH secretion to 65.6% of control levels. The lowest effective dose of 3HP that significantly inhibited (by 31%) LHRH-induced FSH secretion was 10(-14) M 3HP. Maximum suppression by 3HP of basal FSH secretion occurred between 10(-10)-10(-8) M, and maximum suppression of LHRH-induced secretion occurred at 10(-12) M. None of the other gonadal steroids tested (progesterone, testosterone, ...Continue Reading

Citations

Sep 1, 1990·The Journal of Steroid Biochemistry and Molecular Biology·J P WiebeF Hertelendy
Nov 30, 1990·The Journal of Steroid Biochemistry and Molecular Biology·J S Campbell, H J Karavolas
Apr 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·J P WiebeK D Buckingham
May 2, 2002·The Journal of Steroid Biochemistry and Molecular Biology·Felicia V Nowak
Jan 6, 2000·The Journal of Steroid Biochemistry and Molecular Biology·W P EechauteJ M Kaufman
Dec 16, 2006·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Darhl Foreman

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