Selective suppression of lymphokine production by human hybridoma suppressor factor (HSF)

Immunobiology
Y TomitaM K Cathcart

Abstract

We have made a human thymus cell hybridoma that secretes an immunosuppressive monoclonal lymphokine, referred to as hybridoma suppressor factor (HSF). This factor modulates the function of CD4+ cells suppressing their IL-2 production and suppressing PWM-induced B cell differentiation into Ig producing cells. Here we have examined the effect of HSF on the generation of T cell-derived lymphokines that regulate B cell growth and differentiation as well as the expression of other proteins involved in the control of T cell growth i.e., the p55 chain of the IL-2R and the transferrin receptor (TFR). HSF suppressed IFN-gamma activity produced by mitogen-stimulated PBMC without affecting the generation of lymphokines responsible for BCGF and BCDF activities. Additionally, HSF did not inhibit the expression of either IL-2R (p55) or TFR by activated T cells in spite of causing the suppression of IL-2 production. This evidence was further supported by experiments in which HSF selectively suppressed the accumulation of IL-2 mRNA without affecting IL-2R (p55) mRNA expression in mitogen-stimulated PBMC. The selective action of HSF may help to clarify the regulatory mechanisms involved in lymphokine gene expression as well as provide a way by ...Continue Reading

References

Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T HiranoT Kishimoto
Nov 1, 1988·Immunopharmacology·Y TomitaM K Cathcart
Aug 1, 1984·The Journal of Experimental Medicine·M TsudoH Uchino
Aug 26, 1982·Nature·P W Gray, D V Goeddel
Jun 4, 1984·Nature·H J LeibsonJ W Kappler

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