Selective tethering of ligands and proteins to a microfluidically patterned electroactive fluid lipid bilayer array

Langmuir : the ACS Journal of Surfaces and Colloids
Debjit DuttaMuhammad N Yousaf

Abstract

We report a new, quantitative methodology to pattern and present ligands from planar, supported, fluid lipid bilayers. By combining microfluidic lithography (microFL) with an electroactive, chemoselective interfacial reaction strategy, a number of ligands as well as protein concanavalin A were immobilized in lipid microarrays. Electroactive vesicles were generated after the spontaneous insertion of hydroquinone-tethered alkane (H(2)Q) into egg palmitoyl-oleoyl phosphatidylcholine (egg-POPC), followed by subsequent fusion to a siloxane-terminated self-assembled monolayer (SAM) on gold. An advantage of the H(2)Q system is that it can be electrochemically oxidized to the corresponding quinone (Q), followed by rapid chemoselective conjugation with oxyamine-functionalized (RONH(2)) ligands. The oxime product is also electroactive, and the reaction can be monitored and the amount of ligand bound can be quantified by electrochemistry. The bilayers were characterized by electrochemistry, fluorescence microscopy, and ellipsometry and were determined to be fluid by fluorescence recovery after photobleaching (FRAP). This strategy provides a synergistic method to pattern and present a number of ligands or biomolecules from the bilayer surf...Continue Reading

References

Jan 1, 1981·The Journal of Membrane Biology·L T BoniS W Hui
Dec 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·J T GrovesH M McConnell
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·J Israelachvili
Mar 14, 2002·Journal of the American Chemical Society·Ye FangJoydeep Lahiri
Apr 17, 2003·Journal of the American Chemical Society·Tinglu YangPaul S Cremer
Aug 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Raghuveer Parthasarathy, Jay T Groves
Aug 25, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Kenichi MorigakiTakahisa Taguchi
Aug 16, 2005·Prostaglandins & Other Lipid Mediators·Li Feng
Nov 19, 2005·Science·Kaspar D MossmanMichael L Dustin
Jan 5, 2006·Journal of the American Chemical Society·Adrian M BrozellAtul N Parikh
Feb 4, 2006·Chembiochem : a European Journal of Chemical Biology·Jwa-Min NamJay T Groves
Mar 28, 2006·Nature Materials·Tae-Young YoonSin-Doo Lee
May 3, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Bradley P HarrisAndrew T Metters
Nov 30, 2006·Journal of the American Chemical Society·Eugene W L Chan, Muhammad N Yousaf
Jun 9, 2007·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Eugene W L Chan, Muhammad N Yousaf
Feb 9, 2008·Journal of the American Chemical Society·Jinjun ShiPaul S Cremer
Feb 19, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Nathan P Westcott, Muhammad N Yousaf
May 16, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Sungjin Park, Muhammad N Yousaf
Jul 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Brian M LambMuhammad N Yousaf
Jul 26, 2008·Nature Nanotechnology·Xinjian ZhouPaul L McEuen
Aug 30, 2008·Chembiochem : a European Journal of Chemical Biology·Brian M LambMuhammad N Yousaf
Sep 30, 2008·Chembiochem : a European Journal of Chemical Biology·Brian M LambMuhammad N Yousaf

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Citations

Feb 16, 2013·Analytical and Bioanalytical Chemistry·Frank MeinersGunther Wittstock
Dec 8, 2011·Analytical Chemistry·Irep Gözen, Aldo Jesorka
Nov 8, 2011·Bioconjugate Chemistry·Debjit DuttaMuhammad N Yousaf
May 13, 2011·Journal of the American Chemical Society·Debjit DuttaMuhammad N Yousaf
Aug 27, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew K Strulson, Joshua A Maurer
Sep 1, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew K Strulson, Joshua A Maurer
May 23, 2013·Chemistry : a European Journal·Ying ZhangXiaojun Han
Jan 20, 2017·Organic & Biomolecular Chemistry·Radhakrishnan Mohan RajDeivanayagam Easwaramoorthy
Nov 25, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Aleksandra MarkovicGunther Wittstock
Aug 14, 2020·Bioconjugate Chemistry·Isaac W MoranDavid J D Carter

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