PMID: 11330814May 2, 2001Paper

Selective 'unlabeling' of amino acids in fractionally 13C labeled proteins: an approach for stereospecific NMR assignments of CH3 groups in Val and Leu residues

Journal of Biomolecular NMR
Hanudatta S Atreya, Kandala V R Chary

Abstract

A novel methodology for stereospecific NMR assignments of methyl (CH3) groups of Val and Leu residues in fractionally 13C-labeled proteins is presented. The approach is based on selective 'unlabeling' of specific amino acids in proteins while fractionally 13C-labeling the rest. A 2D [13C-1H] HSQC spectrum recorded on such a sample is devoid of peaks belonging to the 'unlabeled' amino acid residues. Such spectral simplification aids in unambiguous stereospecific assignment of diastereotopic CH3 groups in Val and Leu residues in large proteins. This methodology has been demonstrated on a 15 kDa calcium binding protein from Entamoeba histolytica (Eh-CaBP).

Citations

Jul 12, 2012·Journal of Biomolecular NMR·Garima JaipuriaHanudatta S Atreya
Dec 15, 2015·Chembiochem : a European Journal of Chemical Biology·Abhinav DubeyHanudatta S Atreya
Mar 10, 2006·Journal of Computational Chemistry·Primoz Pristovsek, Lorella Franzoni
Apr 8, 2010·Quarterly Reviews of Biophysics·Masatsune Kainosho, Peter Güntert
Nov 29, 2015·Journal of Biomolecular NMR·Rafal AugustyniakFabien Ferrage
Sep 15, 2010·Journal of Biomolecular NMR·Garima JaipuriaHanudatta S Atreya
Dec 15, 2010·Journal of Biomolecular NMR·B KrishnarjunaHanudatta S Atreya
Mar 13, 2012·Chembiochem : a European Journal of Chemical Biology·Rodolfo M RasiaMichael J Plevin
Sep 5, 2012·Journal of Biomolecular NMR·David Pantoja-Uceda, Jorge Santoro
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Paola TuranoIvano Bertini
Mar 5, 2020·Progress in Nuclear Magnetic Resonance Spectroscopy·Stefan Schütz, Remco Sprangers
Jul 21, 2004·Biochemistry·Sourajit M MustafiClaudio Luchinat
Aug 5, 2004·Journal of the American Chemical Society·Vitali Tugarinov, Lewis E Kay
Jan 14, 2009·Journal of Molecular Biology·Ravi Pratap BarnwalKandala V R Chary

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