Selective uptake of cholesteryl ester from high density lipoproteins by plasma membranes of adipose tissue

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
J G Parkes, A Angel

Abstract

The interaction between high density lipoproteins (HDL) and adipose tissue is an important pathway for cholesterol and cholesteryl ester flux. In intact fat cells, a disproportionately greater net uptake of cholesteryl ester occurs subsequent to lipoprotein binding than would have been predicted from a consideration of holoparticle uptake alone. To characterize the early events in this process, cholesteryl hexadecyl ether, a nonmetabolizable, accumulative marker of cholesteryl ester, was incorporated into canine HDL2, and its uptake by omental adipocyte plasma membranes was measured in relation to the binding of HDL2, which in this animal species is enriched in apolipoprotein A-I and free of apolipoprotein E. The dose-response profile for HDL2 binding was consistent with a single lipoprotein binding site at all concentrations of HDL2, whereas uptake of cholesteryl ester from HDL2 was biphasic, suggesting a high affinity site at low HDL2 concentrations and a low affinity site at high lipoprotein concentrations. Pronase treatment stimulated binding twofold and this was accompanied by a parallel twofold stimulation of cholesteryl ester uptake. EDTA, on the other hand, reduced binding and uptake of cholesteryl ester by 20%, indicat...Continue Reading

Citations

Sep 1, 1991·Journal of Cellular Physiology·C C ParrishA Angel
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·X ZhaF R Maxfield
Mar 20, 1998·The Journal of Clinical Endocrinology and Metabolism·S AzharE Reaven
Feb 24, 1993·Biochimica Et Biophysica Acta·F RinningerE Windler
Oct 1, 1991·Biochimica Et Biophysica Acta·W J JohnsonM C Phillips

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