Selectively detecting attomolar concentrations of proteins using gold lined nanopores in a nanopore blockade sensor.

Chemical Science
Yanfang WuJ Justin Gooding

Abstract

Disease diagnosis at earlier stages requires the development of ultrasensitive biosensors for detecting low-abundance biomarkers in complex biological fluids within a reasonable time frame. Here, we demonstrate the development of an ultrasensitive nanopore blockade biosensor that can rapidly diagnose a model protein biomarker, prostate-specific antigen (PSA) with high selectivity. The solid-state nanopores have gold located only along the length of the nanopore whilst the rest of the membrane is silicon nitride. The orthogonal use of materials allows nanopore arrays with a different surface chemistry inside the nanopore relative to the rest of the membrane to be fabricated. The importance of this differential surface chemistry is it can improve the detection limit of nanopore blockade sensors in quantitative analysis. Based on such functionalized nanopore devices, nanopore blockade sensors lower the limit of detection by an order of magnitude and enable ultrasensitive detection of PSA as low as 80 aM. The findings from this study open new opportunities for nanopore sensors in further developments including optical detection and ultralow detection limit biosensing at complex biological fluids.

References

Oct 1, 1975·The Journal of General Physiology·J E Hall
Feb 1, 1968·The Journal of General Physiology·B Hille
Jul 15, 2003·Nature Materials·A J StormC Dekker
Sep 2, 2004·Journal of the American Chemical Society·Zuzanna SiwyCharles R Martin
Apr 7, 2005·Journal of the American Chemical Society·Zuzanna SiwyCharles R Martin
Nov 30, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·C Chad HarrellCharles R Martin
May 17, 2007·Nano Letters·Meni Wanunu, Amit Meller
Jan 31, 2008·Chemical Reviews·Matthew E StewartRalph G Nuzzo
Jul 26, 2008·Nature Nanotechnology·Cees Dekker
Apr 8, 2009·Nature Nanotechnology·James ClarkeHagan Bayley
Jul 23, 2009·Chemical Society Reviews·Stefan Howorka, Zuzanna Siwy
Nov 27, 2009·Nature·David A Giljohann, Chad A Mirkin
Feb 5, 2010·Analytical Chemistry·Magnus P JonssonFredrik Höök
Feb 22, 2011·Nature Nanotechnology·Erik C YuskoMichael Mayer
Jun 21, 2011·Nature Nanotechnology·Stefan W KowalczykCees Dekker
Jul 7, 2011·Nanotechnology·Stefan W KowalczykCees Dekker
Sep 6, 2011·Nature Nanotechnology·Yong WangLi-Qun Gu
Mar 13, 2012·Nature Nanotechnology·Ruoshan WeiUlrich Rant
Jun 5, 2012·Physics of Life Reviews·Meni Wanunu
Mar 13, 2015·ACS Applied Materials & Interfaces·Mateu Pla-RocaErik Reimhult
Jul 23, 2016·Angewandte Chemie·J Justin Gooding, Katharina Gaus
Sep 25, 2017·Advances in Colloid and Interface Science·Mathilde LepoitevinSebastien Balme
Nov 23, 2018·Journal of the American Chemical Society·Yanfang WuJ Justin Gooding
May 10, 2019·Nature Communications·Kyloon ChuahJ Justin Gooding
Jun 28, 2019·Nanotechnology·Alessio FragassoCees Dekker
Oct 1, 2019·Advanced Materials·Yanfang WuJohn Justin Gooding
Oct 12, 2019·Nanoscale·Olivia M EggenbergerMichael Mayer
Feb 13, 2020·ACS Nano·Alessio FragassoCees Dekker

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Methods Mentioned

BETA
chips
scanning
transmission
electron
electron beam lithography
dot array
biosensor
transmission electron microscopy
electron imaging
energy

Software Mentioned

ImageJ

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