Selectivity in the mechanism of action of antimicrobial mastoparan peptide Polybia-MP1.

European Biophysics Journal : EBJ
Marcia Perez Dos Santos CabreraJoão Ruggiero Neto

Abstract

Many potent antimicrobial peptides also present hemolytic activity, an undesired collateral effect for the therapeutic application. Unlike other mastoparan peptides, Polybia-MP1 (IDWKKLLDAAKQIL), obtained from the venom of the social wasp Polybia paulista, is highly selective of bacterial cells. The study of its mechanism of action demonstrated that it permeates vesicles at a greater rate of leakage on the anionic over the zwitterionic, impaired by the presence of cholesterol or cardiolipin; its lytic activity is characterized by a threshold peptide to lipid molar ratio that depends on the phospholipid composition of the vesicles. At these particular threshold concentrations, the apparent average pore number is distinctive between anionic and zwitterionic vesicles, suggesting that pores are similarly formed depending on the ionic character of the bilayer. To prospect the molecular reasons for the strengthened selectivity in Polybia-MP1 and its absence in Mastoparan-X, MD simulations were carried out. Both peptides presented amphipathic alpha-helical structures, as previously observed in Circular Dichroism spectra, with important differences in the extension and stability of the helix; their backbone solvation analysis also indi...Continue Reading

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Citations

Oct 8, 2013·European Biophysics Journal : EBJ·Luciana Moro Puia ZaninJoão Ruggiero Neto
Mar 7, 2014·European Biophysics Journal : EBJ·Natália Bueno LeiteJoão Ruggiero Neto
Oct 12, 2015·Computational Biology and Chemistry·Mariya A ToropovaAndrey A Toropov
Aug 26, 2015·Toxicon : Official Journal of the International Society on Toxinology·Nathalia Batista DiasMario Sergio Palma
Apr 28, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dinara AliverdievaChristophor Sholtz
Jun 12, 2013·Toxicon : Official Journal of the International Society on Toxinology·D BaracchiG Moneti
Jul 16, 2016·Toxicon : Official Journal of the International Society on Toxinology·Jacqueline GonçalvesMárcia Mortari
Apr 6, 2019·Scientific Reports·Timothée RivelSemen Yesylevskyy
Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Davor JuretićFrançois-Xavier Pellay
Oct 28, 2017·Archives of Pharmacal Research·Huy X LuongYoung-Woo Kim
Oct 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Anindya BasuSusanna Su Jan Leong
Mar 19, 2021·Journal of the American Society for Mass Spectrometry·Christian IeritanoW Scott Hopkins
May 2, 2018·Toxicon : Official Journal of the International Society on Toxinology·José Roberto Aparecido Dos Santos-PintoMario Sergio Palma
Jun 6, 2021·Journal of Colloid and Interface Science·Danubia Batista MartinsMarcia Perez Dos Santos Cabrera
Jul 30, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marcia P dos Santos CabreraJoão Ruggiero Neto

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