Selectivity of interaction of phospholipids with bovine spinal cord myelin basic protein studied by spin-label electron spin resonance.

Biochemistry
M B SankaramD Marsh

Abstract

The selectivity of interaction between bovine spinal cord myelin basic protein (MBP) and eight different spin-labeled lipid species in complexes with dimyristoylphosphatidylglycerol (DMPG) and between spin-labeled phosphatidylglycerol and spin-labeled phosphatidylcholine in complexes of MBP with various mixtures of DMPG and dimyristoylphosphatidylcholine (DMPC) has been studied by electron spin resonance (ESR) spectroscopy. In DMPC/DMPG mixtures, the protein binding gradually decreased with increasing mole fraction of DMPC in a nonlinear fashion. The lipid-protein binding assays indicated a preferential binding of the protein to phosphatidylglycerol relative to phosphatidylcholine without complete phase separation of the two lipids. The outer hyperfine splittings (2Amax) of both phosphatidylglycerol and phosphatidylcholine labeled at C-5 of the sn-2 chain (5-PGSL and 5-PCSL, respectively) were monitored in the lipid-protein complexes as a function of the mole fraction of DMPC. The increases in the value of Amax induced on binding of the protein were larger for 5-PGSL than for 5-PCSL, up to 0.25 mole fraction of DMPC. Beyond this mole fraction the spectral perturbations induced by the protein were similar for both lipid labels. ...Continue Reading

References

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Citations

Nov 20, 1993·Biotechnology and Bioengineering·B D KelleyT A Hatton
Jan 1, 1991·The International Journal of Biochemistry·T Cserháti, M Szögyi
Sep 10, 2014·Molecular Therapy. Nucleic Acids·Guido Tomás RozenblumAlfredo Daniel Vitullo
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Aug 1, 1990·FEBS Letters·D Marsh
Aug 2, 2008·Methods : a Companion to Methods in Enzymology·Derek Marsh
Feb 26, 2008·Biochimica Et Biophysica Acta·Derek Marsh
Aug 1, 2009·Biochemistry·George HarauzJoan M Boggs
May 4, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Katharina WidderDariush Hinderberger

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