Selectivity of odorant-binding proteins from the southern house mosquito tested against physiologically relevant ligands

Frontiers in Physiology
Jiao YinWalter S Leal

Abstract

As opposed to humans, insects rely heavily on an acute olfactory system for survival and reproduction. Two major types of olfactory proteins, namely, odorant-binding proteins (OBPs) and odorant receptors (ORs), may contribute to the selectivity and sensitivity of the insects' olfactory system. Here, we aimed at addressing the question whether OBPs highly enriched in the antennae of the southern house mosquito, Culex quinquefasciatus, contribute at least in part to the selective reception of physiologically relevant compounds. Using a fluorescence reporter and a panel of 34 compounds, including oviposition attractants, human-derived attractants, and repellents, we measured binding affinities of CquiOBP1, CquiOBP2, and CquiOBP5. Based on dissociation constants, we surmised that CquiOBP2 is a carrier for the oviposition attractant skatole, whereas CquiOBP1 and CquiOBP5 might transport the oviposition pheromone MOP, a human-derived attractant nonanal, and the insect repellent picardin. Binding of these three ligands to CquiOBP1 was further analyzed by examining the influence of pH on apparent affinity as well as by docking these three ligands into CquiOBP1. Our findings suggest that CquiOBP1 might discriminate MOP from nonanal/pica...Continue Reading

References

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Aug 22, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Karl-Ernst Kaissling
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Dec 23, 2014·Current Opinion in Insect Science·Walter S Leal

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Citations

Aug 19, 2016·Cellular and Molecular Life Sciences : CMLS·Christina E DrakouSpyros E Zographos
Sep 6, 2020·Applied Microbiology and Biotechnology·Nathália F BritoAna Claudia A Melo
Sep 14, 2018·Frontiers in Physiology·Daniele S OliveiraAna C A Melo
Nov 2, 2019·Journal of Medical Entomology·Johan Sebastián Portilla-PulidoStelia Carolina Mendez-Sanchez

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Methods Mentioned

BETA
PCR
ion-exchange chromatography
fluorescence
RNA-Seq
X-ray
nuclear magnetic resonance

Software Mentioned

MOP
Sybyl
- Dock
Surflex
CScore
GraphPad
Prism
Chimera

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