Selegiline, an MAO-B inhibitor, attenuates airway smooth muscle contraction in the rat trachea

The Journal of Pharmacy and Pharmacology
Maki YoshimuraK Sumikawa

Abstract

Selegiline is widely used for Parkinson's disease and sometimes for Alzheimer's disease. It is reported to affect intracellular Ca(2+) concentration. Since intracellular Ca(2+) is partly regulated by phosphatidylinositol (PI) response and is important for smooth muscle contraction, selegiline may affect airway smooth muscle tension. We examined the effects of selegiline on acetylcholine (ACh)- and KCl-induced contractile and PI responses in rat trachea. The trachea was cut into 3-mm-wide ring segments or 1-mm-wide slices. ACh (3 microM, 50% effective dose) or KCl (40 mM) was added, and ring relaxation was induced by the addition of selegiline. Tracheal slices were incubated with [(3)H]myo-inositol and 3 microM ACh in the presence of selegiline, and [(3)H]inositol monophosphate (IP(1)) was measured. Selegiline dose-dependently attenuated ACh- and KCl-induced tracheal ring contractions. Fifty-percent inhibitory doses (ID50) of selegiline against ACh- and KCl-induced contraction were 120 +/- 30 microM and 80 +/- 20 microM, respectively. Basal and ACh-induced IP(1) accumulation were 2.20 +/- 0.20 Bq and 7.88 +/- 0.23 Bq, respectively, and selegiline at a dose of 1000 microM attenuated ACh-induced IP(1) accumulation (5.44 +/- 0.30 B...Continue Reading

References

Feb 1, 1988·Archives of Biochemistry and Biophysics·G E KassS Orrenius
Sep 1, 1993·Trends in Pharmacological Sciences·U Quast
Aug 1, 1997·Clinical Pharmacokinetics·I Mahmood
Feb 24, 2001·The Journal of Pharmacy and Pharmacology·O ShibataK Sumikawa
Jan 29, 2002·Journal of Neuroscience Research·M EbadiH El Refaey

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Citations

Jan 26, 2010·American Journal of Respiratory Cell and Molecular Biology·Taher NassarAbd Al-Roof Higazi
Nov 23, 2011·Phytotherapy Research : PTR·Grace G L YuePing-Chung Leung

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