PMID: 11925459Apr 2, 2002Paper

Selenite and selenomethionine promote HL-60 cell cycle progression

The Journal of Nutrition
Huawei Zeng

Abstract

The essential role of selenium (Se) in nutrition is well established. The elucidation of the mechanisms by which selenium regulates the cell cycle can lead to a better understanding of the nature of selenium's essentiality and its role in disease prevention. In this study, the effects of selenium deficiency or adequacy (0.25 micromol/L selenite or selenomethionine) on HL-60 cell cycle progression were examined in serum-free media. Selenium was critical for promotion of HL-60 cell growth. Cell-cycle analysis revealed that selenium deficiency caused a decrease in G1 phase cells that corresponded to an increase in G2 and sub-G1 phase cells. Gene array analysis suggested that c-Myc, cyclin C, proliferating cell nuclear antigen, cyclin-dependent kinase (cdk)1, cdk2, cdk4, cyclin B and cyclin D2 mRNA levels were lower in selenium-deficient cells than in the cells supplemented with 0.25 micromol/L selenomethionine. The decrease in the c-Myc mRNA level in selenium-deficient cells was confirmed by reverse transcription-polymerase chain reaction analysis. Furthermore, the phosphorylation state of total cellular protein was higher (57%) in selenium-supplemented cells than in selenium-deficient cells. Collectively, these results suggest a ...Continue Reading

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Citations

Mar 28, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huawei Zeng
Jul 31, 2003·The Journal of Biological Chemistry·Yoshiro SaitoEtsuo Niki
Sep 21, 2006·International Journal of Urology : Official Journal of the Japanese Urological Association·Eliane KellenFrank Buntinx
Jan 27, 2005·The Journal of Nutrition·Huawei Zeng, Mary Briske-Anderson
Apr 24, 2009·Biological Trace Element Research·Wayne Chris HawkesKevin Dawson
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Feb 27, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Zi-wei ZhangShi-wen Xu
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Jan 5, 2014·Chemical Research in Toxicology·Simona ConstantinescuNiko Beerenwinkel

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