Selenite assimilation into formate dehydrogenase H depends on thioredoxin reductase in Escherichia coli

Journal of Biochemistry
Muneaki TakahataKenji Inagaki

Abstract

Escherichia coli growing under anaerobic conditions produce H(2) and CO(2) by the enzymatic cleavage of formate that is produced from pyruvate at the end of glycolysis. Selenium is an integral part of formate dehydrogenase H (FDH H), which catalyses the first step in the formate hydrogen lyase (FHL) system. The genes of FHL system are transcribed only under anaerobic conditions, in the presence of a sigma 54-dependent transcriptional activator FhlA that binds formate as an effector molecule. Although the formate addition to the nutrient media has been an established procedure for inducing high FDH H activity, we have identified a low-salt nutrient medium containing <0.1% NaCl enabled constitutive, high expression of FDH H even without formate and d-glucose added to the medium. The novel conditions allowed us to study the effects of disrupting genes like trxB (thioredoxin reductase) or gor (glutathione reductase) on the production of FDH H activity and also reductive assimilation of selenite ( SeO 3(2-)) into the selenoprotein. Despite the widely accepted hypothesis that selenite is reduced by glutathione reductase-dependent system, it was demonstrated that trxB gene was essential for FDH H production and for labelling the FDH H...Continue Reading

Citations

Apr 9, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun LuArne Holmgren
Sep 1, 2009·Proteome Science·Ashley JasenecJonathan Caguiat
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Takashi TamuraKenji Inagaki
Mar 13, 2014·Applied Microbiology and Biotechnology·Kien Trung TranThomas K Wood
Feb 9, 2012·Critical Reviews in Biochemistry and Molecular Biology·Karen TrchounianArmen Trchounian
Sep 18, 2015·The Journal of Biological Chemistry·Ivan R SilvaOtavio H Thiemann
Mar 26, 2013·The Journal of Biological Chemistry·Tetsu M IshiiSergey V Steinberg
May 1, 2021·Metallomics : Integrated Biometal Science·Michael WellsJohn F Stolz
Mar 31, 2009·Biochimica Et Biophysica Acta·Satoko Yoshizawa, August Böck

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