Abstract
Aflatoxin B1 (AFB1) can cause hepatotoxicity, genotoxicity, and immunosuppressive effects for a variety of organisms. Selenium (Se), as an essential nutrient element, plays important protective effects against cell apoptosis induced by AFB1. This research aimed to reveal the ameliorative effects of selenium on AFB1-induced excess apoptosis in chicken splenocytes through death receptor and endoplasmic reticulum pathways in vivo. Two hundred sixteen neonatal chickens, randomized into four treatments, were fed with basal diet (control treatment), 0.4 mg/kg Se supplement (+Se treatment), 0.6 mg/kg AFB1 (AFB1 treatment), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se (AFB1 + Se treatment) during 21 days of experiment, respectively. Compared with the AFB1 treatment, the levels of splenocyte apoptosis in the AFB1 + Se treatment were obviously dropped by flow cytometry and TUNEL assays although they were still significantly higher than those in the control or + Se treatments. Furthermore, the mRNA expressions of CASP-3, CASP-8 and CASP-10, GRP78, GRP94, TNF-α, TNF-R1, FAS, and FASL of splenocytes in the AFB1 + Se treatment by qRT-PCR assay were significantly decreased compared with the AFB1 treatment. These results indicate that Se could partially ...Continue Reading
References
Nov 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R D DoradoT M Aquino
Aug 1, 1967·Food and Cosmetics Toxicology·I F Purchase
Jan 1, 1994·Critical Reviews in Eukaryotic Gene Expression·E LittleA S Lee
Aug 26, 1998·Immunology Today·R C McKenzieG J Beckett
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 16, 2002·Gut·M MergerK Croitoru
Mar 11, 2004·Mycopathologia·K K Jakhar, J R Sadana
Aug 2, 2005·Toxicology·P B WangikarA G Telang
Aug 2, 2005·Nature Reviews. Immunology·Reina E Mebius, Georg Kraal
Apr 4, 2008·Molecular Nutrition & Food Research·Peter R Hoffmann, Marla J Berry
Apr 17, 2008·Nature Reviews. Molecular Cell Biology·Alexei Degterev, Junying Yuan
Sep 22, 2011·Biological Trace Element Research·Dayou ShiZhaoxin Tang
Nov 10, 2011·Biological Trace Element Research·Shining GuoZhaoxin Tang
Nov 10, 2011·Toxins·Agha W YunusJosef Bohm
Mar 20, 2013·Journal of Applied Toxicology : JAT·Guoqing QianJia-Sheng Wang
Jul 11, 2013·International Journal of Environmental Research and Public Health·Fengyuan WangWeimin Lai
Sep 7, 2013·Environmental Toxicology and Pharmacology·Keke MiaoZigui Zhang
Oct 8, 2013·Toxicology and Industrial Health·Jin ChenYi Geng
Feb 13, 2014·International Journal of Environmental Research and Public Health·Kejie ChenQingqiu Yang
Jun 10, 2014·Biological Trace Element Research·Yang HeLi Tang
Aug 21, 2014·International Journal of Environmental Research and Public Health·Xi PengKejie Chen
Sep 28, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kejie ChenYi Zhou
Apr 17, 2015·BMC Cancer·Sophie BustanyBrigitte Sola
Feb 27, 2016·Advances in Cancer Research·Bill X WuZihai Li
Mar 5, 2016·Oncotarget·Xi PengShan Zhou
May 10, 2017·Biological Trace Element Research·Jing FangJunliang Deng
Jun 25, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yaqi DaiWentao Xu
Sep 15, 2017·Oncotarget·Chunyu LiuYu Sun
Oct 4, 2017·Nature Reviews. Nephrology·Andrey V Cybulsky
Nov 23, 2017·Oncotarget·Zhixiang ZhengSong Zhao
Dec 17, 2017·Oncotarget·Panpan ZhuXici Liu