Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro

International Journal of Reproductive Medicine
Jennifer A TolenP J Chan

Abstract

Objectives. Human papillomaviruses (HPV) are associated with cell cycle arrest. This study focused on antioxidant selenomethionine (SeMet) inhibition of HPV-mediated necrosis. The objectives were to determine HPV-18 effects on embryonic cells and to evaluate SeMet in blocking HPV-18 effects. Methods. Fertilized mouse embryos were cultured for 5 days to implanted trophoblasts and exposed to either control medium (group 1), HPV-18 (group 2), combined HPV-18 and 0.5 µM SeMet (group 3), or combined HPV-18 and 5.0 µM SeMet (group 4). After 48 hrs, trophoblast integrity and, apoptosis/necrosis were assessed using morphometric and dual-stain fluorescence assays, respectively. Results. HPV-18 exposed trophoblasts nuclei (253.8 ± 28.5 sq·µ) were 29% smaller than controls (355.6 ± 35.9 sq·µ). Supplementation with 0.5 and 5.0 µM SeMet prevented nuclear shrinkage after HPV-18 exposure. HPV-18 infected trophoblasts remained larger with SeMet supplementation. HPV-18 decreased cell viability by 44% but SeMet supplementation sustained cell viability. Apoptosis was lower when SeMet was present. HPV-18 decreased inner cell mass (ICM) viability by over 60%. Conclusions. HPV-18 decreased nuclear size and trophoblast viability but these effects wer...Continue Reading

References

Jan 1, 1992·Gynäkologisch-geburtshilfliche Rundschau·M ManaviE Reinold
Aug 27, 1971·The Journal of Organic Chemistry·R Walter, J Roy
Jul 31, 1999·Fertility and Sterility·P J ChanA King
May 16, 2003·American Journal of Obstetrics and Gynecology·Sara C RowlandPhilip J Chan
Oct 31, 2008·Acta Obstetricia Et Gynecologica Scandinavica·Marja E SarkolaStina M Syrjänen
Nov 5, 2008·Cancer·Martin A WhitesideElizabeth R Unger
May 13, 2009·Infectious Agents and Cancer·Philip E Castle
Jul 16, 2011·American Journal of Clinical Pathology·Zhuang ZuoJ Elliot Carter
Oct 22, 2011·Free Radical Biology & Medicine·Aldwin Suryo Rahmanto, Michael J Davies
Jan 12, 2013·Archives of Gynecology and Obstetrics·Linda J HongPhilip J Chan
Sep 10, 2013·BMC Pregnancy and Childbirth·GeumJoon ChoHaiJoong Kim
Oct 8, 2013·American Journal of Obstetrics and Gynecology·Mollie McDonnoldMaged M Costantine
Jan 21, 2014·Cancer Biomarkers : Section a of Disease Markers·Agnieszka M MazurekE Małusecka
Mar 15, 2014·Reproductive Health·Hannah BlencoweUNKNOWN Born Too Soon Preterm Birth Action Group
Sep 18, 2014·Archives of Gynecology and Obstetrics·Sarah S ChenPhilip J Chan
Dec 3, 2014·Biochimica Et Biophysica Acta·Aristi P Fernandes, Valentina Gandin
Dec 24, 2014·Biochimica Et Biophysica Acta·Cesira FoppoliM Perluigi

❮ Previous
Next ❯

Citations

Mar 24, 2021·Cancer Control : Journal of the Moffitt Cancer Center·Ewa JablonskaTong Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence

Software Mentioned

Adobe Photoshop
Excel

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

The Journal of Obstetrics and Gynaecology of the British Commonwealth
P V Taylor, K W Hancock
European Journal of Obstetrics, Gynecology, and Reproductive Biology
Kaylene R CarrPhilip J Chan
© 2021 Meta ULC. All rights reserved