Selenium-binding protein 2, the major hepatic target for acetaminophen, shows sex differences in protein abundance

Electrophoresis
Jens MattowJoachim Klose

Abstract

Liver samples from female and male mice of two subspecies, Mus musculus musculus and Mus musculus domesticus, were investigated by a combination of 2-DE and MALDI-MS. The image analysis of the generated 2-DE patterns revealed several protein spots with significant differences in intensity/abundance between the sexes. Seven protein spots, which were prominent in 2-DE patterns of male mice, but which showed very low intensities in females, were identified as selenium-binding protein 2 (SBP2) also known as 56-kDa acetaminophen-binding protein. Edman degradation indicated that at least three of these protein spots represent N-terminally truncated SBP2 variants. Furthermore, it was shown that the observed differences in SBP2 abundance correlate with sex differences in transcription of the gene encoding SBP2, selenbp2, as revealed by RT-PCR and restriction digest as well as sequence analysis of the products. Since SBP2 has been described as the major target for acetaminophen in mouse liver cytosol, these findings are discussed with respect to their possible relevance for sex differences in acetaminophen-mediated toxicity, which have been described in a variety of mammals including mice and rats.

References

Feb 1, 1992·Rapid Communications in Mass Spectrometry : RCM·B SpenglerE Jaeger
Feb 14, 1992·Biochemical and Biophysical Research Communications·N R PumfordJ A Hinson
Jan 1, 1991·Advances in Experimental Medicine and Biology·R B BirgeE A Khairallah
Nov 1, 1989·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J B BartoloneE A Khairallah
May 1, 1989·Toxicology and Applied Pharmacology·W P BeierschmittS D Cohen
Mar 1, 1967·European Journal of Biochemistry·P Edman, G Begg
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D C DahlinS D Nelson
Jan 1, 1995·Drug Metabolism Reviews·J A HinsonD W Roberts
Aug 31, 1993·Biochemical and Biophysical Research Communications·P JamesG Gonnet
Sep 1, 1993·Toxicology and Applied Pharmacology·J J HuC S Yang
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J HenzelC Watanabe
Jul 1, 1996·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R C Blantz
Mar 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J B TarloffR S Goldstein
Jul 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·D J HoivikS D Cohen
Mar 1, 1996·Chemical Research in Toxicology·N C HalmesN R Pumford
Mar 1, 1997·Toxicology and Applied Pharmacology·S D CohenJ A Hinson
Feb 1, 1997·Drug Metabolism Reviews·S D Cohen, E A Khairallah
Oct 17, 1998·Toxicology and Applied Pharmacology·H ZaherF J Gonzalez
Dec 3, 1999·Journal of Clinical Pharmacy and Therapeutics·E Tanaka
Nov 20, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Laura P JamesJack A Hinson
Feb 5, 2004·Proteomics·Ji Young ParkYoung-Ki Paik
Jul 1, 2004·Molecular and Cellular Biology·Ruiyin ChuMatthew Davison
Jun 1, 1993·Current Biology : CB·D J PappinA J Bleasby
Sep 21, 2004·Rapid Communications in Mass Spectrometry : RCM·Wen-Hai JinRong Zeng
Oct 19, 2004·The American Journal of Emergency Medicine·William A WatsonDouglas Borys

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Citations

Jun 6, 2009·Journal of Toxicology and Environmental Health. Part a·Xi ChenNai-jun Tang
Mar 25, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Amy L SilversAndrew C Chang
Jun 3, 2014·Genome Biology·Luz D OrozcoMatteo Pellegrini
Mar 23, 2010·The Journal of Nutritional Biochemistry·Irina A KirpichIon V Deaciuc
May 12, 2009·Biochemical Pharmacology·Jofre Tenorio-LarangaJ Arturo García-Horsman
May 23, 2006·Mechanisms of Ageing and Development·James M HarperRichard A Miller
Apr 16, 2014·Autism Research and Treatment·Laura J RaymondNicholas V C Ralston
Jun 29, 2011·Toxicology and Applied Pharmacology·Xiuzhen FanM Firoze Khan

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