Selenium blocks porcine circovirus type 2 replication promotion induced by oxidative stress by improving GPx1 expression

Free Radical Biology & Medicine
Xingxiang ChenKehe Huang

Abstract

Porcine circovirus type 2 (PCV2) is recognized as a key infectious agent in postweaning multisystemic wasting syndrome (PMWS), but not all pigs infected with PCV2 will develop PMWS. The aim of this work was to explore the relationships among PCV2 infection, oxidative stress, and selenium in a PK-15 cell culture model of PCV2 infection. The results showed that oxidative stress induced by H(2)O(2) treatment increased PCV2 replication as measured by PCV2 DNA copies and the number of infected cells. Furthermore, PCV2 replication was inhibited by selenomethionine (SeMet) at a high concentration (6μM) and the increase in PCV2 replication by oxidative stress was blocked by SeMet at physiological concentrations (2 or 4μM). PCV2 infection caused a decrease in glutathione peroxidase 1 (GPx1) activity but an increase in GPx1 mRNA levels, suggesting that GPx1 may represent an important defense mechanism during PCV2 infection. SeMet did not significantly block the promotion of PCV2 replication in GPx1-knockdown cells. This observation correlates with the observed influence of SeMet on GPx1 mRNA and activity in GPx1-knockdown cells, indicating that GPx1 plays a key role in blocking the promotion of PCV2 replication. We conclude that differen...Continue Reading

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Citations

Oct 2, 2013·International Journal of Biological Macromolecules·Zi-Jie SuTing-Jun Hu
Jan 31, 2013·Journal of Agricultural and Food Chemistry·Fang GanKehe Huang
Jan 16, 2014·Biological Trace Element Research·Fahmida ParveenKehe Huang
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Aug 8, 2020·The British Journal of Nutrition·Giovanna BermanoJohn E Hesketh
May 4, 2017·BMC Complementary and Alternative Medicine·Hai-Lan ChenTing-Jun Hu

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