Selenium deficiency inhibits differentiation and immune function and imbalances the Th1/Th2 of dendritic cells

Metallomics : Integrated Biometal Science
Zhepeng SunShu Li

Abstract

Selenium (Se) deficiency inhibits immune cell differentiation, affects immune response, and leads to cellular and humoral immune dysfunction. However, the impact of Se deficiency on the differentiation and Th1/Th2 balance of dendritic cells is still unclear. In this study, we replicated a model of Se-deficient chickens by feeding the chickens with a low-Se diet (i.e., the content of Se is 0.008 mg per kg diet). On this basis, we explored the effect of Se deficiency on the differentiation of chicken dendritic cells by induction culture of peripheral blood monocyte cells. We induced chicken dendritic cells by incubating mononuclear cells with a 100 ng mL-1 recombinant chicken granulocyte-macrophage colony-stimulating factor and 20 ng mL-1 recombinant chicken IL-4 for total 7 days. The results showed that Se deficiency decreased the expression of cell-surface markers including CD11c, CD40, CD86, and MHC II. Furthermore, we analyzed the cytokine profiles using real-time quantitative PCR and ELISA. The results indicated that Se deficiency inhibited the expression of selenoproteins and changed the secretion of IL-10, IL-12p40, and IFN-γ. Additionally, Se deficiency weakened the ability of dendritic cells to stimulate the proliferatio...Continue Reading

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Citations

Jun 16, 2018·Metallomics : Integrated Biometal Science·Rui-Feng FanShi-Wen Xu
Oct 24, 2018·Metallomics : Integrated Biometal Science·Jianfa WangRui Wu
May 31, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Ibrahim Elmadfa, Alexa L Meyer
Dec 14, 2019·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Zhe LiuRui Wu
Nov 22, 2019·Biological Trace Element Research·Yilei ZhangShiwen Xu
Sep 25, 2020·Metallomics : Integrated Biometal Science·Yafan GongZiwei Zhang
Jun 30, 2019·Oxidative Medicine and Cellular Longevity·Yiming ZhangShu Li
Dec 20, 2018·Metallomics : Integrated Biometal Science·Jingzeng CaiZiwei Zhang
Dec 6, 2018·Journal of Cellular Physiology·Yafan GongZiwei Zhang
May 5, 2021·Immunity, Inflammation and Disease·Tatjana ZakeIlze Konrade
Aug 27, 2021·Biological Trace Element Research·Jia-Hong ChuRui-Feng Fan
Oct 3, 2021·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Yi JiaZhu Zeng
Nov 18, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Monika MichalczukPaweł Konieczka
Sep 12, 2020·Research in Veterinary Science·Jia-Hong ChuRui-Feng Fan

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Methods Mentioned

BETA
PCR
flow cytometry
reverse-transcription PCR
ELISA

Software Mentioned

GraphPad Prism
Flowjo
Premier

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