PMID: 8954158Nov 15, 1996Paper

Selenium-dependent peroxidases suppress 5-lipoxygenase activity in B-lymphocytes and immature myeloid cells. The presence of peroxidase-insensitive 5-lipoxygenase activity in differentiated myeloid cells

European Journal of Biochemistry
Oliver Werz, Dieter Steinhilber

Abstract

Differentiation of HL-60 cells by dimethylsulfoxide induces 5-lipoxygenase protein expression, but only low cellular 5-lipoxygenase activity. Similarly, B-lymphocytes express 5-lipoxygenase protein and show activity in cell homogenates but not in intact cells. Here, we demonstrate that suppression of cellular 5-lipoxygenase activity in these cell lines is serum dependent and that the serum effect can be mimicked by selenium. Selenium-dependent inhibition of 5-lipoxygenase activity was also observed in the corresponding cell homogenates or 100,000 x g supernatants when dithiothreitol or glutathione (GSH) was added. The properties of the endogenous selenium-dependent inhibitor, i.e., molecular mass, utilization of GSH and dithiothreitol as substrates, sensitivity to iodacetate, inhibition of 5-lipoxygenase activity in the presence of the GPx-1 inhibitor mercaptosuccinate, suggest that a selenoenzyme with properties of the phospholipid hydroperoxide glutathione peroxidase (GPx-4) is responsible for the 5-lipoxygenase inhibition in BL41-E95-A and immature HL-60 cells. Differentiation of HL-60 cells in the presence of 1,25-dihydroxyvitamin D3 and transforming growth factor-beta (TGF beta) upregulated cellular 5-lipoxygenase activity...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P J JakobssonB Samuelsson
Oct 15, 1989·The Biochemical Journal·D RiendeauD J Nathaniel
Nov 16, 1987·European Journal of Biochemistry·A Hatzelmann, V Ullrich
Oct 1, 1988·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W F StensonZ Bar-Shavit
Nov 30, 1988·Biochemical and Biophysical Research Communications·A J van den Eijnden-van RaaijE J van Zoelen
Jul 1, 1987·Chemistry and Physics of Lipids·F Ursini, A Bindoli
Jan 1, 1988·Annals of the New York Academy of Sciences·C A RouzerH G Bull
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C A Rouzer, B Samuelsson
Mar 29, 1985·Biochimica Et Biophysica Acta·F UrsiniC Gregolin
Oct 30, 1981·Biochemical and Biophysical Research Communications·R W BonserP Cuatrecasas
Jul 29, 1983·Biochemical and Biophysical Research Communications·S YalçinB Mannervik
Jan 1, 1981·Methods in Enzymology·A Wendel
Dec 30, 1994·Biochemical and Biophysical Research Communications·M BrungsD Steinhilber
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·M BrungsD Steinhilber
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SumimotoK Takeshige
Jun 30, 1993·Biochemical and Biophysical Research Communications·D SteinhilberB Samuelsson
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SteinhilberB Samuelsson
Jul 28, 1993·Biochimica Et Biophysica Acta·D SteinhilberB Samuelsson
Mar 15, 1996·The Journal of Biological Chemistry·R A LepleyF A Fitzpatrick

❮ Previous
Next ❯

Citations

Oct 3, 2002·Journal of Clinical Laboratory Analysis·Huriye Kuzu-KarsilayanGülderen Yanikkaya-Demirel
Nov 26, 2002·Biochemical Pharmacology·Dana AlbertOliver Werz
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Olof Rådmark
Oct 29, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J Chaudière, R Ferrari-Iliou
Jan 11, 2003·Free Radical Biology & Medicine·Hirotaka Imai, Yasuhito Nakagawa
Sep 3, 2004·Nature Reviews. Cancer·Steven A Belinsky
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·O WerzO Rådmark
Sep 4, 2010·Antioxidants & Redox Signaling·Susan J Fairweather-TaitRachel Hurst
Oct 29, 2009·Carcinogenesis·Christopher M LyonSteven A Belinsky
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·O P Rådmark
Jan 13, 2009·BMC Immunology·Yilmaz MahshidHans-Erik Claesson
Apr 14, 2007·Environmental Health Perspectives·Denis GanycWilliam T Self
Nov 5, 2011·Biological Chemistry·Ann-Kathrin HäfnerDieter Steinhilber
Oct 26, 2001·Biochemical and Biophysical Research Communications·T GaschottJ Stein
Nov 12, 2013·Antioxidants & Redox Signaling·Khader AwwadIngrid Fleming
Jan 22, 2010·Cellular and Molecular Life Sciences : CMLS·Svenja D SteinbrinkThorsten J Maier
Dec 29, 2015·European Journal of Medicinal Chemistry·Antonella PedutoRosanna Filosa
Dec 6, 2011·Biochimica Et Biophysica Acta·Michael HörnigDieter Steinhilber
Aug 12, 2008·Biochemical Pharmacology·Thorsten J MaierGerd Geisslinger
Jun 16, 2004·British Journal of Pharmacology·Lutz FischerOliver Werz
Nov 3, 2007·European Journal of Haematology·Gudmundur RunarssonHans-Erik Claesson
Feb 21, 2004·Biochemical and Biophysical Research Communications·Jagadananda Ghosh
May 24, 2005·Biochemical Pharmacology·Oliver Werz, Dieter Steinhilber
Jul 14, 2006·Pharmacology & Therapeutics·Oliver Werz, Dieter Steinhilber
May 2, 2006·Molecular and Cellular Endocrinology·Etty OsherNaftali Stern
Aug 27, 2005·Biochemical and Biophysical Research Communications·Olof Rådmark, Bengt Samuelsson
May 9, 2000·Biochemical Pharmacology·O WerzD Steinhilber
Sep 4, 2015·Biochimica Et Biophysica Acta·Ann-Kathrin HäfnerDieter Steinhilber
Jul 27, 2015·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Yilmaz MahshidHans-Erik Claesson
Apr 22, 2015·Journal of Immunological Methods·Olga SchererStefan Lorkowski
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Maria Teresa Rizzo
Jun 18, 2004·Journal of Clinical Laboratory Analysis·Eda Imren-EryilmazAyse Ogan
Oct 11, 2005·Journal of Cellular Biochemistry·Thomas G Brock

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.