Seleno-Chitosan induces apoptosis of lung cancer cell line SPC-A-1 via Fas/FasL pathway.

Bioorganic Chemistry
Peng WuAn-Jun Liu

Abstract

In this study, human lung cancer SPC-A-1 cells were cultured with Seleno-Chitosan to study the mechanism of apoptosis. CCK-8 results showed that with the increase of the concentration of Seleno-Chitosan and the prolongation of culture time, the inhibition on the proliferation of SPC-A-1 cells gradually increased, and the morphology of SPC-A-1 cells changed obviously. Flow cytometry result suggested that the concentration of calcium ion, level of reactive oxygen species and mitochondrial membrane potential increased. Western blot showed that Seleno-Chitosan induced apoptosis via the mitochondrial pathway in SPC-A-1. Eventually, we found 15 proteins that were expressed abnormally by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), which were all related to Fas/FasL pathway of cells.

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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