Selenocysteine-Specific Mass Spectrometry Reveals Tissue-Distinct Selenoproteomes and Candidate Selenoproteins

Cell Chemical Biology
Lin GuoYaoyang Zhang

Abstract

Selenoproteins, defined by the presence of selenocysteines (Sec), play important roles in a wide range of biological processes. All known selenoproteins are marked by the presence of Sec insertion sequence (SECIS) at their mRNA. The lack of an effective analytical method has hindered our ability to explore the selenoproteome and new selenoproteins beyond SECIS. Here, we develop a Sec-specific mass spectrometry-based technique, termed "SecMS," which allows the systematic profiling of selenoproteomes by selective alkylation of Sec. Using SecMS, we quantitatively characterized the age- and stress-regulated selenoproteomes for nine tissues from mice of different ages and mammalian cells, demonstrating tissue-specific selenoproteomes and an age-dependent decline in specific selenoproteins in brains and hearts. We established an integrated platform using SecMS and SECIS-independent selenoprotein (SIS) database and further identified five candidate selenoproteins. The application of this integrated platform provides an effective strategy to explore the selenoproteome independent of SECIS.

Citations

May 2, 2019·Molecular & Cellular Proteomics : MCP·Steven VerbruggenGerben Menschaert
Aug 15, 2019·Antioxidants & Redox Signaling·Ling FuJing Yang
May 3, 2020·Proceedings of the National Academy of Sciences of the United States of America·Mark P JedrychowskiBruce M Spiegelman
Oct 13, 2020·FEMS Microbiology Ecology·Michael Wells, John F Stolz
Aug 9, 2019·Molecular & Cellular Proteomics : MCP·Steven VerbruggenGerben Menschaert
Feb 23, 2021·Frontiers in Neuroscience·Nikolay SolovyevBernhard Michalke
Mar 19, 2021·Frontiers in Neuroscience·Ulrich SchweizerNoelia Fradejas-Villar
Apr 10, 2021·Comprehensive Reviews in Food Science and Food Safety·Minhao XieYong Fang
Apr 12, 2019·Journal of the American Society for Mass Spectrometry·Arvind M KorwarK Sandeep Prabhu
Jul 25, 2021·International Journal of Molecular Sciences·Jordan SonetLaurent Chavatte
Sep 17, 2019·Accounts of Chemical Research·Jennifer C Peeler, Eranthie Weerapana

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