Self-assembled β-lactoglobulin-oleic acid and β-lactoglobulin-linoleic acid complexes with antitumor activities

Journal of Dairy Science
Bing FangF Z Ren

Abstract

β-Lactoglobulin (β-LG) can bind to fatty acids such as oleic acid (OA) and linoleic acid (LA). Another whey protein, α-lactalbumin (α-LA), can also bind to OA to give the complex α-LA-OA, which has antitumor properties. Based on reports that the activity of α-LA-OA is highly dependent on OA, as well as the acquisition of similar complexes using other proteins, such as lysozyme and lactoferrin, we speculated whether β-LG could also kill tumor cells after binding to other fatty acids. Therefore, we prepared complexes of β-LG with OA (β-LG-OA) and LA (β-LG-LA) in the current study and evaluated them in terms of antitumor activity and thermostability using the methylene blue method and differential scanning calorimetry, respectively. The structural features of these complexes were also evaluated using fluorescence spectroscopy and circular dichroism. The binding dynamics of OA and LA to β-LG were studied using isothermal titration calorimetry. Cell viability results revealed that β-LG-LA and β-LG-OA exhibited similar antitumor activities. Interestingly, the binding of β-LG to LA led to an increase in its thermostability, whereas its binding to OA had very little effect. The environments of the tryptophan residues in the β-LG-OA and...Continue Reading

References

Dec 29, 1998·The Journal of Biological Chemistry·S Y WuL Sawyer
Aug 10, 2000·Protein Science : a Publication of the Protein Society·L RagonaH Molinari
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·D Leckband
Jul 24, 2003·Protein Science : a Publication of the Protein Society·Maddalena ColliniGiancarlo Baldini
Oct 13, 2005·Journal of Molecular Recognition : JMR·Abdessamad Ababou, John E Ladbury
Nov 22, 2005·Biochimica Et Biophysica Acta·Fang TianLorenzo Brancaleon
Feb 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·O HallgrenC Svanborg
May 9, 2006·Biochemical and Biophysical Research Communications·Jenny PetterssonCatharina Svanborg
Sep 29, 2006·Journal of Molecular Recognition : JMR·Adessamad Ababou, John E Ladbury
Aug 4, 2007·Journal of the American Chemical Society·Mrinmoy DeVincent M Rotello
Sep 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kazumasa Sakurai, Yuji Goto
Jan 18, 2008·Journal of Molecular Recognition : JMR·Oksana Okhrimenko, Ilian Jelesarov
Dec 3, 2008·International Journal of Cancer. Journal International Du Cancer·Sonja AitsCatharina Svanborg
Nov 19, 2009·Biological Chemistry·Alberto BarbiroliMaria Caterina Vilardo
Aug 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Vladana VukojevićLudmilla A Morozova-Roche
Jan 21, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·T Sanjoy Singh, Sivaprasad Mitra
Apr 8, 2011·Journal of Biomolecular Structure & Dynamics·Maliheh S AtriAmir Niasari-Naslaji
Apr 22, 2011·Journal of Agricultural and Food Chemistry·Alberto BarbiroliStefania Iametti
May 6, 2011·The Journal of Physical Chemistry. B·Imed HasniHeidar-Ali Tajmir-Riahi
Dec 26, 2013·Biochimica Et Biophysica Acta·Bing FangFa Zheng Ren

❮ Previous
Next ❯

Citations

Jan 30, 2021·Journal of Biomolecular Structure & Dynamics·Manik N WaghmarePrabhakar M Dongre
Jan 30, 2021·Food Research International·Julieta N NasoAna M R Pilosof

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis