PMID: 9545583May 16, 1998Paper

Self-assembled alpha-hemolysin pores in an S-layer-supported lipid bilayer

Biochimica Et Biophysica Acta
B SchusterU B Sleytr

Abstract

The effects of a supporting proteinaceous surface-layer (S-layer) from Bacillus coagulans E38-66 on a 1,2-diphytanoyl-sn-glycero-3-phosphatidylcholine (DPhPC) bilayer were investigated. Comparative voltage clamp studies on plain and S-layer supported DPhPC bilayers revealed no significant difference in the capacitance. The conductance of the composite membrane decreased slightly upon recrystallization of the S-layer. Thus, the attached S-layer lattice did not interpenetrate or rupture the DPhPC bilayer. The self-assembly of a pore-forming protein into the S-layer supported lipid bilayer was examined. Staphylococcal alpha-hemolysin formed lytic pores when added to the lipid-exposed side. The assembly was slow compared to unsupported membranes, perhaps due to an altered fluidity of the lipid bilayer. No assembly could be detected upon adding alpha-hemolysin monomers to the S-layer-faced side of the composite membrane. Therefore, the intrinsic molecular sieving properties of the S-layer lattice do not allow passage of alpha-hemolysin monomers through the S-layer pores to the lipid bilayer. In comparison to plain lipid bilayers, the S-layer supported lipid membrane had a decreased tendency to rupture in the presence of alpha-hemoly...Continue Reading

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Citations

Apr 7, 2006·Analytical and Bioanalytical Chemistry·Andreas Janshoff, Claudia Steinem
Feb 27, 1999·Current Opinion in Biotechnology·H Bayley
Jan 6, 2001·Journal of Biotechnology·B Schuster, U B Sleytr
Jan 12, 2002·Bioelectrochemistry·Bernhard Schuster, Uwe B Sleytr
Jun 1, 2008·Biointerphases·Bernhard SchusterUwe B Sleytr
Nov 20, 2012·The European Physical Journal. E, Soft Matter·Frédéric HarbBernard Tinland
Jan 29, 2013·International Journal of Molecular Sciences·Dietmar PumUwe B Sleytr
May 9, 2014·Journal of the Royal Society, Interface·Bernhard Schuster, Uwe B Sleytr
Jan 31, 2015·International Journal of Molecular Sciences·Samar DamiatiBernhard Schuster
Sep 24, 2004·IEEE Transactions on Nanobioscience·Bernhard SchusterUwe B Sleytr
Jul 9, 2003·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·U B SleytrD Pum
Apr 21, 1999·Biochimica Et Biophysica Acta·C MaderU B Sleytr
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
May 16, 2006·Biochimica Et Biophysica Acta·Markus SondermannJan C Behrends
Mar 1, 2007·FEMS Microbiology Letters·Uwe B SleytrEva M Egelseer
Feb 16, 2006·IEE Proceedings. Nanobiotechnology·D PumU B Sleytr
Sep 15, 2001·Nature·H Bayley, P S Cremer
Dec 23, 2016·Proceedings of the National Academy of Sciences of the United States of America·Matthias GartenGilman E S Toombes
May 2, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Peter J BeltramoJan Vermant
May 27, 2004·Journal of the American Chemical Society·Matthew A HoldenPaul S Cremer
Mar 24, 2009·Journal of Structural Biology·Bernhard Schuster, Uwe B Sleytr

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