Self-Assembled Arginine-Capped Peptide Bolaamphiphile Nanosheets for Cell Culture and Controlled Wettability Surfaces

Biomacromolecules
Emerson Rodrigo da SilvaI W Hamley

Abstract

The spontaneous assembly of a peptide bolaamphiphile in water, namely, RFL4FR (R, arginine; F, phenylalanine; L, leucine) is investigated, along with its novel properties in surface modification and usage as substrates for cell culture. RFL4FR self-assembles into nanosheets through lateral association of the peptide backbone. The L4 sequence is located within the core of the nanosheets, whereas the R moieties are exposed to the water at the surface of the nanosheets. Kinetic assays indicate that the self-assembly is driven by a remarkable two-step process, where a nucleation phase is followed by fast growth of nanosheets with an autocatalysis process. The internal structure of the nanosheets is formed from ultrathin bolaamphiphile monolayers with a crystalline orthorhombic symmetry with cross-β organization. We show that human corneal stromal fibroblast (hCSF) cells can grow on polystyrene films coated with films dried from RFL4FR solutions. For the first time, this type of amphiphilic peptide is used as a substrate to modulate the wettability of solid surfaces for cell culture applications.

References

May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 1, 1997·Journal of Biomedical Materials Research·M LampinM F Sigot-Luizard
Jul 19, 2000·Biochimica Et Biophysica Acta·L C Serpell
Nov 23, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·G PabstP Laggner
Jan 25, 2002·Nature·Michael Zasloff
Jun 6, 2003·Journal of the American Chemical Society·Kun LuDavid G Lynn
Jul 30, 2003·Biophysical Journal·Hans Binder, Göran Lindblom
Jun 10, 2004·Chemical Reviews·Jürgen-Hinrich Fuhrhop, Tianyu Wang
Nov 8, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eric Vives
Mar 16, 2006·Journal of the American Chemical Society·Jijun DongDavid G Lynn
Mar 26, 2008·Chemical Society Reviews·Rein V Ulijn, Andrew M Smith
Dec 17, 2008·Biochimica Et Biophysica Acta·Aimee M MorrisRichard G Finke
Nov 21, 2009·FEBS Letters·Nathan SchmidtGerard C L Wong
Jul 3, 2010·Chemical Society Reviews·Roberto de la Rica, Hiroshi Matsui
Jun 23, 2012·Accounts of Chemical Research·Shuguang Zhang
Aug 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yuichi YoshimuraYuji Goto
Jan 5, 2013·The Journal of Physical Chemistry. B·Michelle S LiberatoWendel A Alves
Jan 12, 2013·Angewandte Chemie·Kyle L MorrisLouise C Serpell
Jan 17, 2013·Molecular Pharmaceutics·Roanne R JonesIan W Hamley
Jan 31, 2013·Chemical Communications : Chem Comm·Ian W HamleyValeria Castelletto
Aug 15, 2013·Journal of Physics. Condensed Matter : an Institute of Physics Journal·J E Gillam, C E MacPhee
Jan 1, 2013·Faraday Discussions·Valeria CastellettoIan W Hamley
Aug 12, 2014·Chemical Communications : Chem Comm·Sibaprasad MaityMeital Reches
Mar 24, 2015·Chemical Communications : Chem Comm·Ian W Hamley
Apr 1, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Carla C DecandioWendel A Alves
Jun 23, 2015·Chemical Communications : Chem Comm·Emerson Rodrigo da SilvaIan William Hamley
Nov 28, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ricardo M GouveiaChe J Connon

❮ Previous
Next ❯

Citations

Jun 28, 2016·Bioconjugate Chemistry·Ian W Hamley, Valeria Castelletto
Sep 17, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Ian W HamleyJanne Ruokolainen
May 12, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yueying CaoJiqian Wang
Nov 27, 2019·International Journal of Molecular Sciences·Sungeun LeeChongsuk Ryou
Feb 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lucas R MelloEmerson R Silva
Dec 13, 2016·Physical Chemistry Chemical Physics : PCCP·B M SoaresW A Alves
Jul 14, 2018·Nanomaterials·Wenjing HuangSheng Zhang
Sep 28, 2018·Journal of Biomedical Materials Research. Part a·Yimeng NiuYubo Fan
Sep 1, 2016·Nanotechnology·Melis Sardan EkizMustafa O Guler
Jan 28, 2020·Current Protein & Peptide Science·Sudha ShankarRajkishor Rai
Nov 11, 2019·Advanced Drug Delivery Reviews·Fitsum Feleke SahleTao L Lowe
Nov 11, 2020·The Journal of Chemical Physics·Phuong VoClifford E Woodward
Jun 12, 2019·Bioconjugate Chemistry·Margaret L DalyRonit Freeman
Nov 10, 2018·ACS Omega·Guilherme ColherinhasEudes Eterno Fileti
Dec 17, 2016·Biomacromolecules·Ian W HamleyJoanna Stasiak
Mar 14, 2019·ACS Applied Materials & Interfaces·Xiang LiTao Yi
Jun 4, 2019·ACS Applied Bio Materials·Charlotte J C Edwards-GayleJanne Ruokolainen
Feb 21, 2019·ACS Applied Materials & Interfaces·Valeria CastellettoJanne Ruokolainen
Jun 2, 2018·Bioconjugate Chemistry·Jessica A HutchinsonJanne Ruokolainen
Jul 31, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Valeria CastellettoJanne Ruokolainen
Jul 23, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Antara Dasgupta, Debapratim Das
May 23, 2017·Acta Biomaterialia·Karla E Inostroza-BritoAlvaro Mata
Nov 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fang LeiMingying Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.