Self-assembled multilayer of gold nanoparticles for amplified electrochemical detection of cytochrome c

The Analyst
Jing ZhaoGenxi Li

Abstract

Although different kinds of film materials and some modification techniques are applied for the development of protein-film electrochemistry, the design of a more ordered adsorption platform with improved sensitivity is still required. Here we employ single-strand DNA (ssDNA)-functionalized gold nanoparticles as scaffolds for the construction of a multilayered uniform self-assembled structure via the hybridization of complementary ssDNA. After adsorbing with native conformation onto the uniformly built electrode, cytochrome c responded very well in voltammetry experiments. The peak currents increase with the addition of the number of gold nanoparticle layers, which indicates that the multilayer gold nanoparticles not only provide a compatible microenvironment for the protein to undergo direct electron transfer reactions but also amplify the electrochemical signals by increasing the binding sites for the protein immobilization. Furthermore, ultra-sensitive detection of cytochrome c by using this multilayer gold nanoparticle-modified electrode is carried out. The linear range is from 2 x 10(-9) to 1 x 10(-7) M with a detection limit of 6.7 x 10(-10) M.

References

Sep 8, 2000·Science·T A TatonR L Letsinger
Jun 6, 2006·Bioelectrochemistry·Itamar WillnerEugenii Katz
Aug 21, 2007·Journal of the American Chemical Society·Paolo BertoncelloPatrick R Unwin

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Citations

Sep 5, 2009·Analytical and Bioanalytical Chemistry·Fallyn W Campbell, Richard G Compton
Jan 1, 2010·Sensors·Khalid M Abu-SalahAnees Ahmad Ansari
Dec 12, 2012·Bioelectrochemistry·Manickam PandiarajChandran Karunakaran
Sep 15, 2009·Biosensors & Bioelectronics·Sebastian Markus Bonk, Fred Lisdat
Sep 14, 2016·Biosensors & Bioelectronics·Pandiaraj ManickamShekhar Bhansali
Mar 6, 2013·Chemical Communications : Chem Comm·Eugene MahonMihail Barboiu
Aug 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Morgan L VargoMichael C Leopold
Jul 25, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Michael Riskin, Itamar Willner

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