Self-assembled nanowire arrays as three-dimensional nanopores for filtration of DNA molecules

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Sakon RahongYoshinobu Baba

Abstract

Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.

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Citations

Nov 7, 2015·Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B·Peter G ShanklesScott T Retterer
Aug 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiangling LiFanmao Liu
Jun 13, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xiaoyin SunYoshinobu Baba
Mar 20, 2021·Physical Review. E·Luhui NingKe Chen

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