PMID: 11322878Apr 27, 2001Paper

Self-assembled pearling structure of long duplex DNA with histone H1

European Journal of Biochemistry
Y YoshikawaK Yoshikawa

Abstract

We report that complexes of giant DNA molecules with histone H1 proteins form a pearl necklace-like structure when the complexes are prepared by natural dilution from a high-salt solution (2 M NaCl) to a low-salt solution (0.2 M and 50 mM NaCl). We performed real-time observations on the conformational changes of individual T4 phage DNA (166 kb) molecules in bulk solution by fluorescence microscopy. To identify H1-binding regions on individual DNA molecules, we also performed immunofluorescence microscopic observations on the DNA-H1 complex spread on a glass surface. It was found that histone H1 binds DNA in a highly co-operative manner and is accompanied by local folding of the DNA. On the basis of the experimental observations and a theoretical simulation, we propose a self-assembling mechanism for the pearling structure.

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Citations

Dec 1, 2002·Journal of Biological Physics·K Yoshikawa
Jul 9, 2003·European Journal of Biochemistry·Yuko YoshikawaKenichi Yoshikawa
Jun 2, 2011·Biomicrofluidics·Rolf DootzThomas Pfohl
Dec 16, 2011·The Journal of Chemical Physics·Yuko YoshikawaTadayuki Imanaka
Mar 3, 2005·The Journal of Chemical Physics·Naomi MiyazawaRaoul Zana
Sep 16, 2006·Biopolymers·Vladimir BudkerJon A Wolff
Jun 21, 2008·Molecular Biology Reports·Annekathrin HaberlandWolfgang Henke
Mar 8, 2020·Biophysics Reviews·Kanta TsumotoKenichi Yoshikawa
Oct 4, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·T Iwaki, K Yoshikawa
Apr 10, 2003·Journal of the American Chemical Society·Anatoly A ZinchenkoKenichi Yoshikawa
Mar 31, 2011·The Journal of Physical Chemistry. B·Naoko MakitaKenichi Yoshikawa
Jan 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Hisanori NakanishiMasahiko Abe

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