Self-assembled pH-responsive hyaluronic acid-g-poly((L)-histidine) copolymer micelles for targeted intracellular delivery of doxorubicin

Acta Biomaterialia
Lipeng QiuDawei Chen

Abstract

Hyaluronic acid (HA) was conjugated with hydrophobic poly(l-histidine) (PHis) to prepare a pH-responsive and tumor-targeted copolymer, hyaluronic acid-g-poly(l-histidine) (HA-PHis), for use as a carrier for anti-cancer drugs. The effect of the degree of substitution (DS) on the pH-responsive behaviour of HA-PHis copolymer micelles was confirmed by studies of particles of different sizes. In vitro drug release studies demonstrated that doxorubicin (DOX) was released from HA-PHis micelles in a pH-dependent manner. In vitro cytotoxicity assays showed that all the blank micelles were nontoxic. However, MTT assay against Michigan Cancer Foundation-7 (MCF-7) cells (overexpressed CD44 receptors) showed that DOX-loaded micelles with a low PHis DS were highly cytotoxic. Cellular uptake experiments revealed that these pH-responsive HA-PHis micelles taken up in great amounts by receptor-mediated endocytosis and DOX were efficiently delivered into cytosol. Moreover, micelles with the lowest DS exhibited the highest degree of cellular uptake, which indicated that the micelles were internalized into cells via CD44 receptor-mediated endocytosis and the carboxylic groups of HA are the active binding sites for CD44 receptors. Endocytosis inhibi...Continue Reading

References

Jul 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Seong LeeYou Han Bae
Dec 16, 2006·The Journal of Biological Chemistry·Hosami Harada, Masaaki Takahashi
Feb 13, 2007·Nature Structural & Molecular Biology·Suneale BanerjiDavid G Jackson
Sep 11, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Seong LeeYou Han Bae
Jan 12, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Haiqing YinYou Han Bae
Jul 16, 2008·International Journal of Pharmaceutics·Christian Wischke, Steven P Schwendeman
Sep 2, 2008·Nature Reviews. Drug Discovery·Mark E DavisDong M Shin
Nov 21, 2009·Acta Biomaterialia·Kwangwon Seo, Dukjoon Kim
Mar 23, 2010·Advanced Drug Delivery Reviews·Vladimir Torchilin
Mar 15, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rong LiuZhongwei Gu
Apr 26, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Glenn D Prestwich
Jun 7, 2011·International Journal of Pharmaceutics·Susanne FredenbergAnders Axelsson
Sep 29, 2011·International Journal of Pharmaceutics·Yanhua LiuZhonggui He
Oct 5, 2011·ACS Nano·Ki Young ChoiJae Hyung Park
Oct 15, 2011·Advanced Drug Delivery Reviews·Arnaud E FelberJean-Christophe Leroux
Oct 22, 2011·Advanced Drug Delivery Reviews·Liu YangWeihong Tan
Jun 1, 2012·ACS Nano·Christian DohmenErnst Wagner
Jun 30, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhonglin Luo, Jianwen Jiang
Jul 18, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yali WuZhiyuan Zhong
Oct 2, 2012·Acta Biomaterialia·Mahrokh DadsetanMichael J Yaszemski
Feb 20, 2013·Biomacromolecules·Gulden Camci-UnalAli Khademhosseini
Mar 5, 2013·Acta Biomaterialia·Viktoriya SokolovaMatthias Epple
May 8, 2013·Acta Biomaterialia·Xiuwen GuanXuesi Chen

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Citations

Mar 20, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvia ArpiccoFranco Dosio
Nov 4, 2015·Advanced Drug Delivery Reviews·Pei Yun TeoYi Yan Yang
Nov 26, 2015·Advanced Drug Delivery Reviews·Franco DosioElias Fattal
Apr 5, 2016·International Journal of Molecular Sciences·Jing-Liang WuZhi-Qin Gao
Apr 30, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Minming WuJianhao Zhao
Oct 9, 2015·International Journal of Cell Biology·Suniti MisraShibnath Ghatak
Apr 5, 2016·ACS Applied Materials & Interfaces·Qian CaiAlideertu Dong
May 3, 2015·Carbohydrate Polymers·Somkamol ManchunPornsak Sriamornsak
Dec 5, 2014·Archives of Toxicology·V Orian-Rousseau, H Ponta
May 24, 2017·Journal of Biomaterials Science. Polymer Edition·Kwanghyok JongShufen Zhang
Feb 9, 2017·Biomaterials·Hyemin KimSei Kwang Hahn
Jul 30, 2015·Biomaterials Science·Shahdeep KaurRinti Banerjee
Aug 25, 2018·Biomaterials Science·Kinga PioreckaWlodzimierz A Stanczyk
May 30, 2019·Drug Development Research·Asghar NarmaniNouraddin Abdi Goushbolagh
Nov 2, 2019·Journal of Biomaterials Science. Polymer Edition·Penghui WangBo Chi
Aug 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jinyu LiWeisan Pan
Apr 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elita MontanariPietro Matricardi
Jan 1, 2016·International Journal of Oncology·Éva KatonaRóza Zákány
Jan 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Huaxia ShiXiaochen Dong
Oct 8, 2016·Nanoscale·Kegang LiuPatrick Hunziker
Aug 24, 2019·Cells·Tzyy-Yue WongPei-Wen Cheng
Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rimesh AugustineIl Kim

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