Self-assembled polyelectrolyte nanoparticles as fluorophore-free contrast agents for multicolor optical imaging

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Da Hye ShinYong Taik Lim

Abstract

In this work, we describe the fabrication of self-assembled polyelectrolyte nanoparticles that provide a multicolor optical imaging modality. Poly(γ-glutamic acid)(γ-PGA) formed self-assembled nanoparticles through electrostatic interactions with two different cationic polymers: poly(L-lysine)(PLL) and chitosan. The self-assembled γ-PGA/PLL and γ-PGA/chitosan nanoparticles were crosslinked by glutaraldehyde. Crosslinking of the ionic self-assembled nanoparticles with glutaraldehyde not only stabilized the nanoparticles but also generated a strong autofluorescence signal. Fluorescent Schiff base bonds (C=N) and double bonds (C=C) were generated simultaneously by crosslinking of the amine moiety of the cationic polyelectrolytes with monomeric glutaraldehyde or with polymeric glutaraldehyde. The unique optical properties of the nanoparticles that resulted from the crosslinking by glutaraldehyde were analyzed using UV/Vis and fluorescence spectroscopy. We observed that the fluorescence intensity of the nanoparticles could be regulated by adjusting the crosslinker concentration and the reaction time. The nanoparticles also exhibited high performance in the labeling and monitoring of therapeutic immune cells (macrophages and dendriti...Continue Reading

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Citations

Jul 5, 2016·Journal of Biomedical Materials Research. Part a·Bo CaiXing-Zhong Zhao
Sep 4, 2018·Chembiochem : a European Journal of Chemical Biology·Shukun LiXuehai Yan
Dec 12, 2020·Scientific Reports·Alessandro CapoMonique Lacroix

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Methods Mentioned

BETA
imaging techniques
fluorescence imaging
fluorescence optical imaging
transmission electron microscopy
Fluorescence
flow cytometry
fluorescence microscopy
dynamic light scattering

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