Self-assembled tethered bimolecular lipid membranes

Advances in Clinical Chemistry
Eva-Kathrin SinnerWolfgang Knoll

Abstract

This chapter describes some of the strategies developed in our group for designing, constructing and structurally and functionally characterizing tethered bimolecular lipid membranes (tBLM). We introduce this platform as a novel model membrane system that complements the existing ones, for example, Langmuir monolayers, vesicular liposomal dispersions and bimolecular ("black") lipid membranes. Moreover, it offers the additional advantage of allowing for studies of the influence of membrane structure and order on the function of integral proteins, for example, on how the composition and organization of lipids in a mixed membrane influence the ion translocation activity of integral channel proteins. The first strategy that we introduce concerns the preparation of tethered monolayers by the self-assembly of telechelics. Their molecular architecture with a headgroup, a spacer unit (the "tether") and the amphiphile that mimics the lipid molecule allows them to bind specifically to the solid support thus forming the proximal layer of the final architecture. After fusion of vesicles that could contain reconstituted proteins from a liposomal dispersion in contact to this monolayer the tethered bimolecular lipid membrane is obtained. Thi...Continue Reading

Citations

May 9, 2014·Journal of the Royal Society, Interface·Bernhard Schuster, Uwe B Sleytr
Apr 12, 2018·Biosensors·Bernhard Schuster
Mar 25, 2020·Sensors·Samar Damiati, Bernhard Schuster
Jan 25, 2013·Molecular Membrane Biology·Nadim ShadiacMaria Hrmova
Aug 27, 2014·Advanced Materials·Patrick O SaboeManish Kumar
Jul 30, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·J BompardA Girard-Egrot
Jan 8, 2013·Journal of the American Chemical Society·Friedrich RoderJacob Piehler
Apr 11, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Stéphanie PaceFrédérique Cunin
Sep 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Meriem ChadliAgnès Girard-Egrot

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